Interspecies reactivity of five monoclonal antibodies to Mycobacterium tuberculosis as examined by immunoblotting and enzyme-linked immunosorbent assay
| Autor: | K. Hasløv, J. Bennedsen, A. B. Andersen, Zeng-Lin Yuan, B. Vergmann |
|---|---|
| Rok vydání: | 1986 |
| Předmět: |
Microbiology (medical)
Tuberculosis medicine.drug_class Enzyme-Linked Immunosorbent Assay Cross Reactions Monoclonal antibody Epitope Mycobacterium Mycobacterium tuberculosis Epitopes Mice Species Specificity Antigen medicine Animals Humans Immunosorbent Techniques Antigens Bacterial Mice Inbred BALB C Mycobacterium bovis biology Antibodies Monoclonal biology.organism_classification medicine.disease Antibodies Bacterial Molecular biology Concanavalin A biology.protein Research Article |
| Zdroj: | Journal of Clinical Microbiology. 23:446-451 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.23.3.446-451.1986 |
| Popis: | Five different murine monoclonal antibodies (MAbs) to Mycobacterium tuberculosis were examined for degree of cross-reactivity with other mycobacterial species by enzyme-linked immunosorbent assay and immunoblotting. One MAb reacted solely with M. tuberculosis and M. bovis BCG. Two of the MAbs reacted with all mycobacterial species examined, whereas two MAbs demonstrated a limited reactivity pattern. The epitopes are located on molecules susceptible to protease treatment, and two of these molecules possess concanavalin A-binding moieties. Two of the antigens defined by these five MAbs are present in tuberculin purified protein derivative. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|