Calcium sensor for photoacoustic imaging
| Autor: | Matthias Preller, Felix Sigmund, Thorsten Bach, Daniel Razansky, Tanja Orschmann, X. Luís Deán-Ben, Vasilis Ntziachristos, Antonella Lauri, Anurag Mishra, Hannes Rolbieski, Panagiotis Symvoulidis, Paul Vetschera, Sheryl Roberts, Yuanyuan Jiang, Anja Stelzl, Sabrina C. Desbordes, Markus Seeger, Gil G. Westmeyer |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Analytical chemistry
chemistry.chemical_element Quantum yield 02 engineering and technology General Chemistry Molar absorptivity Calcium 010402 general chemistry 021001 nanoscience & nanotechnology 01 natural sciences Biochemistry Photobleaching Fluorescence Catalysis 0104 chemical sciences Blueshift Absorbance Colloid and Surface Chemistry chemistry Live cell imaging Biophysics 0210 nano-technology |
| Zdroj: | J. Am. Chem. Soc. 140, 2718-2721 (2018) Journal of the American Chemical Society |
| Popis: | We introduce a selective and cell-permeable calcium sensor for photoacoustics (CaSPA), a versatile imaging technique that allows for fast volumetric mapping of photoabsorbing molecules with deep tissue penetration. To optimize for Ca2+-dependent photoacoustic signal changes, we synthesized a selective metallochromic sensor with high extinction coefficient, low quantum yield, and high photobleaching resistance. Micromolar concentrations of Ca2+ lead to a robust blueshift of the absorbance of CaSPA, which translated into an accompanying decrease of the peak photoacoustic signal. The acetoxymethyl esterified sensor variant was readily taken up by cells without toxic effects and thus allowed us for the first time to perform live imaging of Ca2+ fluxes in genetically unmodified cells and heart organoids as well as in zebrafish larval brain via combined fluorescence and photoacoustic imaging. |
| Databáze: | OpenAIRE |
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