Rapid Detection and Differentiation of Antibodies to HIV-1 and HIV-2 Using Multivalent Antigens and Magnetic Immunochromatography Testing
| Autor: | Holder An, Susan K. Wells, Workman S, Chou-Pong Pau, Owen Sm, Timothy C. Granade |
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| Rok vydání: | 2010 |
| Předmět: |
Microbiology (medical)
Time Factors HIV Antigens Clinical Biochemistry Immunology Human immunodeficiency virus (HIV) HIV Infections HIV Antibodies medicine.disease_cause Immunomagnetic separation Rapid detection Antigen Antibody Specificity medicine Clinical Laboratory Immunology Immunology and Allergy Seroconversion Chromatography medicine.diagnostic_test biology Immunomagnetic Separation business.industry AIDS Serodiagnosis virus diseases Virology Immunoassay HIV-2 HIV-1 biology.protein Antibody Peptides business |
| Zdroj: | Clinical and Vaccine Immunology. 17:1034-1039 |
| ISSN: | 1556-679X 1556-6811 |
| Popis: | A simplified lateral-flow assay for the detection of antibodies to HIV using magnetic-bead conjugates and multibranched peptides from both HIV-1 and HIV-2 was developed. Magnetic immunochromatography testing (MICT) uses a standard lateral-flow platform that incorporates magnetic-bead conjugates for quantitative measurement of the magnetic field distortion associated with the bound magnetic conjugate (reported as adjusted relative magnetic units [MAR]). The results of the optimized MICT assay were compared to standard enzyme immunoassay (EIA) and Western blotting (WB) results using a blinded 649-member panel of specimens from the United States, Cameroon, and West Africa. The panel was comprised of samples from individuals infected with various HIV-1 subtypes ( n = 234) or HIV-2 ( n = 65) and HIV-seronegative specimens ( n = 350). Additionally, 13 HIV-1 seroconversion panels (total specimens = 85), a worldwide panel containing seven of the major circulating HIV-1 subtypes ( n = 18), an HIV-2 panel, an HIV-1/HIV-2 mixed panel, and 100 prospective specimens were tested with completely concordant results. Assay reproducibility (observed MAR) for both intra- and interrun testing was excellent, with coefficients of variation of |
| Databáze: | OpenAIRE |
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