Satellite rats are redundant in embryo-fetal development studies
| Autor: | Manon Beekhuijzen, Harry Emmen, Yaomi de Lange, Mira Wenker, Ankie Lambregts, Birgit Peter |
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| Rok vydání: | 2017 |
| Předmět: |
Male
0301 basic medicine Pathology medicine.medical_specialty Embryonic Development Toxicology Bioinformatics 01 natural sciences Fetal Development 03 medical and health sciences Jugular vein Toxicity Tests medicine Animals Blood Specimen Collection Fetus biology business.industry 010401 analytical chemistry biology.organism_classification Rats 0104 chemical sciences 030104 developmental biology Female Satellite (biology) Jugular Veins business Blood sampling |
| Zdroj: | Reproductive Toxicology. 72:122-128 |
| ISSN: | 0890-6238 |
| DOI: | 10.1016/j.reprotox.2017.06.042 |
| Popis: | Routinely in many laboratories, satellite rats are added to embryo-fetal development (EFD) studies for pharmaceuticals to assess toxicokinetic (TK) properties, because it is assumed that collection of multiple blood samples with relatively large volumes might affect the study outcome. With recent refinement of blood sampling techniques, this belief requires reevaluation. The current work showed successful implementation of jugular vein blood sampling in an EFD rat study without satellite animals, thereby reducing the number of rats in standard EFD studies for pharmaceuticals by 20%. Although not evaluated in this study, microsampling has shown to be very successful and eliminates the need of satellite animals. However, currently not all laboratories have implemented this method and regularly the bioanalytical method is already developed with a limit of quantification that is insufficiently sensitive. Therefore in those cases, a quick win to omit satellite animals can be established by using jugular vein blood sampling. |
| Databáze: | OpenAIRE |
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