Hyperosmotic stress induces cell-dependent aggregation of α-synuclein
Autor: | Fragniere, Alexandra MC, Stott, Simon RW, Fazal, Shaline V, Andreasen, Maria, Scott, Kirsten, Barker, Roger A |
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Přispěvatelé: | Stott, Simon RW [0000-0002-5741-4664], Fazal, Shaline V [0000-0002-2813-4022], Andreasen, Maria [0000-0002-6096-2995], Apollo - University of Cambridge Repository |
Jazyk: | angličtina |
Rok vydání: | 2019 |
Předmět: |
DYNAMICS
Hot Temperature Cell Survival Blotting Western PRION lcsh:Medicine Sodium Chloride Article DISEASE Cell Line Mice FIBRILS FILAMENTS Animals Humans Urea WATER Benzothiazoles lcsh:Science Cell Death L-Lactate Dehydrogenase SECONDARY STRUCTURE lcsh:R Osmolar Concentration Sodium Dodecyl Sulfate Parkinson Disease Hydrogen Peroxide nervous system diseases HEK293 Cells nervous system MUTANT alpha-Synuclein lcsh:Q Electrophoresis Polyacrylamide Gel PROTEIN AGGREGATION KEY ROLE |
Zdroj: | Fragniere, A M C, Stott, S R W, Fazal, S V, Andreasen, M, Scott, K & Barker, R A 2019, ' Hyperosmotic stress induces cell-dependent aggregation of α-synuclein ', Scientific Reports, vol. 9, 2288 . https://doi.org/10.1038/s41598-018-38296-7 Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-11 (2019) |
Popis: | The aggregation of alpha-synuclein (α-syn) is a pathological feature of a number of neurodegenerative conditions, including Parkinson’s disease. Genetic mutations, abnormal protein synthesis, environmental stress, and aging have all been implicated as causative factors in this process. The importance of water in the polymerisation of monomers, however, has largely been overlooked. In the present study, we highlight the role of hyperosmotic stress in inducing human α-syn to aggregate in cells in vitro, through rapid treatment of the cells with three different osmolytes: sugar, salt and alcohol. This effect is cell-dependent and not due to direct protein-osmolyte interaction, and is specific for α-syn when compared to other neurodegeneration-related proteins, such as Tau or Huntingtin. This new property of α-syn not only highlights a unique aspect of its behaviour which may have some relevance for disease states, but may also be useful as a screening test for compounds to inhibit the aggregation of α-syn in vitro. |
Databáze: | OpenAIRE |
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