Studying the potential of upregulated PTGS2 and VEGF-C besides hyper-methylation of PTGS2 promoter as biomarkers of Acute myeloid leukemia

Autor: Masoumeh Kiani-Zadeh, Mohammad Reza Rezvany, Soodeh Namjoo, Mahmood Barati, Mohammad Hossein Mohammadi, Bahare Ghasemi, Tahere Tabatabaei, Ardeshir Ghavamzadeh, Farhad Zaker, Ladan Teimoori-Toolabi
Rok vydání: 2022
Předmět:
Zdroj: Molecular Biology Reports. 49:7849-7862
ISSN: 1573-4978
0301-4851
DOI: 10.1007/s11033-022-07615-7
Popis: Hereby, we aimed to investigate the expression of prostaglandin-endoperoxide synthase 2 (PTGS2) and Vascular Endothelial Factor-C (VEGF-C) besides the methylation of PTGS2 in AML patients. VEGF-C and PTGS2 expression analysis were evaluated in newly diagnosed AML patients and healthy controls by quantitative Reverse Transcriptase PCR method. Also, PTGS2 methylation status was evaluated by Methylation-Sensitive High-Resolution Melting Curve Analysis (MS-HRM). While 34% of patients were female, the mean age of the patients was 43.41 ± 17.60 years suffering mostly from M4 (48.21%) type of AML. Although methylation level between patients and controls was not significantly different, none of the normal controls showed methylation in the PTGS2 promoter. PTGS2 and VEGF-C levels were elevated in AML cases and correlated with WBC, Platelet, and Hemoglobin levels. The survival of patients with overexpressed VEGF-C and PTGS2 was poorer than others. It can be concluded that PTGS2 and especially VEGF-C expression but not PTGS2 methylation can be considered as diagnostic biomarkers for AML.
Databáze: OpenAIRE
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