Phytosphingosine Biosynthesis Differs from Sphingosine in Fish Leukocytes and Involves a Transfer of Methyl Groups from [3H-methyl]Methionine Precursor

Autor:	Georges Zwingelstein, Gérard Brichon, Jacques Portoukalian, Omanand Koul, Jacques Bodennec
Rok vydání:	1998
Předmět:	Niacinamide Chromatography Gas Methionine Sphingosine Phytosphingosine biosynthesis Biophysics Cell Biology Tritium Methylation Biochemistry Serine chemistry.chemical_compound Folic Acid chemistry Leukocytes Animals Moiety Fish Bass Chromatography Thin Layer Molecular Biology Chemical decomposition
Zdroj:	Biochemical and Biophysical Research Communications. 250:88-93
ISSN:	0006-291X
Popis:	We have studied the incorporation of radioactivity from either [3-3H]serine as the direct or [3H-methyl]methionine as the indirect precursor into sphingoid bases of free ceramides in lymphocytes from fish. Radioactivity from serine was incorporated mostly in the sphingosine moiety of ceramides. In contrast, the radioactivity from methionine was exclusively incorporated into phytosphingosine base (i.e., 4-hydroxy-sphinganine) and the incorporation increased by about twofold in the presence of folic acid or niacinamide. Identity of the long-chain bases, phytosphingosine and sphingosine, was established chemically by thin-layer chromatography, chemical degradation, and gas–liquid chromatography.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1bc81d68ac800dd6d5f0fdf2ee339006 https://doi.org/10.1006/bbrc.1998.9273 Zobrazit plný text záznamu Full Text from ScienceDirect