Biocompatibility of designed MicNo-ZnO particles: Cytotoxicity, genotoxicity and phototoxicity in human skin keratinocyte cells
| Autor: | A. Tansu Koparal, Yücel Şahin, Banu Barutca, Ender Suvaci, Uğurcan Özöğüt, Hatice Genç |
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| Přispěvatelé: | Anadolu Üniversitesi, Fen Fakültesi, Biyoloji Bölümü, Koparal, Ayşe Tansu, Şahin, Yücel, Suvacı, Ender |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Keratinocytes Biocompatibility Cell Survival Surface Properties Ultraviolet Rays Cytotoxicity Keratinocyte Cells Nanoparticle Metal Nanoparticles Human skin 02 engineering and technology Toxicology medicine.disease_cause Models Biological Cell Line Phototoxicity 03 medical and health sciences Inhibitory Concentration 50 Oxidants Photochemical medicine Humans Micno-Zno Particle Size Active ingredient Membrane Potential Mitochondrial Chemistry Mutagenicity Tests General Medicine 021001 nanoscience & nanotechnology 3. Good health HaCaT Oxidative Stress 030104 developmental biology Chemical engineering Microscopy Fluorescence Microscopy Electron Scanning Comet Assay Genotoxicity Zinc Oxide 0210 nano-technology Lysosomes Reactive Oxygen Species |
| Zdroj: | Toxicology in vitro : an international journal published in association with BIBRA. 47 |
| ISSN: | 1879-3177 |
| Popis: | WOS: 000426409400025 PubMed ID: 29223573 Recently, designed platelet shaped micron particles that are composed of nano primary particles, called MicNo (= Micron + naNo) particles, have been developed to exploit the benefits of nano size, while removing the adverse effects of nanoparticles. It has been shown that MicNo-ZnO particles exhibit both micron and nanosized particle characteristics. Although physical and chemical properties of MicNo-ZnO particles have been studied, their biocompatibility has not yet been evaluated. Accordingly, the research objective of this study was to evaluate in vitro cytotoxicity, genotoxicity and phototoxicity behaviors of designed MicNo-ZnO particles over human epidermal keratinocyte (HaCaT) cells. MicNo-ZnO particles exhibit much less cytotoxicity with IC50 concentrations between 40 and 50 mu g/ml, genotoxicity above 40 mu g/ml and lower photo genotoxicity under UVA on HaCaT than the ZnO nanoparticles. Although their chemistries are the same, the source of this difference in toxicity values may be attributed to size differences between the particles that are probably due to their ability to penetrate into the cells. In the present study, the expansive and detailed in vitro toxicity tests show that the biocompatibility of MicNo-ZnO particles is much better than that of the ZnO nanoparticles. Consequently, MicNo-ZnO particles can be considered an important active ingredient alternative for sunscreen applications due to their safer characteristics with respect to ZnO nanoparticles. Scientific and Technological Research Council of Turkey [109M585]; Anadolu University Scientific Research Projects Commission [1101F020] This research was supported financially by the Scientific and Technological Research Council of Turkey (Project number: 109M585) and the Anadolu University Scientific Research Projects Commission (Project number: 1101F020). The authors would like to thank Ismail Sahin for his assistance for the UV visible analyses of the nano ZnO and MicNo (R)-ZnO particles. |
| Databáze: | OpenAIRE |
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