Calcium phosphate supplementation increases faecalLactobacillusspp. in a randomised trial of young adults
| Autor: | Amanda L. Ford, Mary C. Christman, Thomas A. Tompkins, W. Combs, Wendy J. Dahl, A. Rohani, Maria Ukhanova, D. Lopez, Volker Mai, Tyler Culpepper, J.A. Coppola |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Adult
Calcium Phosphates Male 0301 basic medicine Microbiology (medical) Adolescent Elemental calcium chemistry.chemical_element Calcium Microbiology law.invention Feces Young Adult 03 medical and health sciences Probiotic 0302 clinical medicine Animal science Double-Blind Method Lactobacillus rhamnosus law Lactobacillus Humans Medicine Cross-Over Studies Lactobacillus helveticus biology Lacticaseibacillus rhamnosus business.industry Probiotics food and beverages Middle Aged biology.organism_classification Crossover study 030104 developmental biology chemistry Dietary Supplements Female 030211 gastroenterology & hepatology business |
| Zdroj: | Beneficial Microbes. 7:3-10 |
| ISSN: | 1876-2891 1876-2883 |
| DOI: | 10.3920/bm2014.0168 |
| Popis: | The aim of the studies was to determine the effects of calcium carbonate and calcium phosphate supplementation on faecal Lactobacillus spp., with and without a probiotic supplement, in healthy adults. Study 1 comprised of a randomised, double-blind, crossover design; participants (n=15) received 2 capsules/d of 250 mg elemental calcium as calcium carbonate (Ca1) and calcium phosphate (Ca2) each for 2-week periods, with 2-week baseline and washout periods. Study 2 was a randomised, double-blind, crossover design; participants (n=17) received 2 capsules/d of Lactobacillus helveticus R0052 and Lactobacillus rhamnosus R0011 (probiotic) alone, the probiotic with 2 capsules/d of Ca1, and probiotic with 2 capsules/d of Ca2 each for 2-week periods with 2-week baseline and washout periods. In both studies, stools were collected during the baseline, intervention and washout periods for Lactobacillus spp. quantification and qPCR analyses. Participants completed daily questionnaires of stool frequency and compliance. In Study 1, neither calcium supplement influenced viable counts of resident Lactobacillus spp., genome equivalents of lactic acid bacteria or stool frequency. In Study 2, faecal Lactobacillus spp. counts were significantly enhanced from baseline when the probiotic was administered with Ca2 (4.83±0.30, 5.79±0.31) (P=0.02), but not with Ca1 (4.98±0.31) or with the probiotic alone (5.36±0.31, 5.55±0.29) (not significant). Detection of L. helveticus R0052 and L. rhamnosus R0011 was significantly increased with all treatments, but did not differ among treatments. There were no changes in weekly stool frequency. Calcium phosphate co-administration may increase gastrointestinal survival of orally-administered Lactobacillus spp. |
| Databáze: | OpenAIRE |
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