Molecular aspects of lipid metabolism in the midgut gland of the brown shrimp Crangon crangon
| Autor: | Reinhard Saborowski, Christoph Held, Wilhelm Hagen, Diana Martínez-Alarcón |
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| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Physiology Crangon Fatty Acid-Binding Proteins 01 natural sciences Biochemistry Arthropod Proteins 03 medical and health sciences Animals 14. Life underwater Molecular Biology Crangonidae chemistry.chemical_classification biology 010604 marine biology & hydrobiology Gene Expression Profiling Crangon crangon fungi Fatty acid Midgut Lipid metabolism biology.organism_classification Lipid Metabolism Shrimp Gastrointestinal Tract Metabolic pathway 030104 developmental biology Fatty acid desaturase chemistry biology.protein lipids (amino acids peptides and proteins) Seasons Transcriptome |
| Zdroj: | EPIC3Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology, 248-24, pp. 110465, ISSN: 10964959 |
| ISSN: | 1879-1107 |
| Popis: | The brown shrimp, Crangon crangon, is well adapted to the variable environmental conditions in the southern North Sea. It is very abundant, has high reproduction rates, and holds a key position in coastal ecosystems. This species has very low lipid deposits in the midgut gland, suggesting that the main function of the midgut gland is metabolic turnover rather than energy storage. Based on seasonal gene expression studies and established transcriptome data, we investigated key components of lipid metabolic pathways. Gene expression of triacylglycerol lipase, phospholipase, and fatty acid desaturase were analyzed and compared with that of other digestive enzymes involved in lipid, carbohydrate, and protein catabolism. Our results suggest that gene expression of digestive enzymes involved in lipid metabolism is modulated by the lipid content in the midgut gland and is related to food availability. Brown shrimp seem to be capable of using cellular phospholipids during periods of food paucity but high energetic (lipid) requirements. Two of three isoforms of fatty acid binding proteins (FABPs) from the midgut gland involved in fatty acid transport showed specific mutations of the binding site. We hypothesize that the mutations in FABPs and deficiencies in anabolic pathways limit lipid storage capacities in the midgut gland of C. crangon. In turn, food utilization, including lipid catabolism, has to be efficient to fulfill the energetic requirements of brown shrimp. |
| Databáze: | OpenAIRE |
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