Cellular Immunity in Current Active Pulmonary Tuberculosis
| Autor: | Beatriz Fernandez, Irma Machado, Romelia Ramirez, Nicolas E. Bianco, Ronald Andrade-Arzabe, Isaac Blanca |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Adult
Male Pulmonary and Respiratory Medicine Cellular immunity CD3 Lymphocyte Activation Peripheral blood mononuclear cell Antigen T-Lymphocyte Subsets In vivo medicine Humans Receptor Tuberculosis Pulmonary Immunity Cellular Lung biology business.industry Receptors Interleukin-2 Middle Aged In vitro medicine.anatomical_structure Immunology Leukocytes Mononuclear biology.protein Interleukin-2 Female business |
| Zdroj: | American Review of Respiratory Disease. 143:496-500 |
| ISSN: | 0003-0805 |
| Popis: | A group of 10 patients with recently diagnosed pulmonary TB were studied and compared to 10 bacillus Calmette-Guérin (BCG) immunized healthy individuals. Cellular immune mechanisms were explored in vitro utilizing fresh and precultured peripheral blood mononuclear cells exposed to PHA, PPD, and recall antigens (SK/SD and CA). Proliferative assays were also carried out in the presence of either each patient's serum (autologous serum) or cocultured with CD3(+)-depleted adherent cells. Serum measurements of soluble interleukin-2 (IL-2) receptor and synthesis of IL-2 generated by mononuclear cells stimulated with PPD and SK/SD were also performed. Patient sera were able to inhibit autologous as well as allogeneic cell responses, and a significant adherent cell suppressive effect was observed. As a whole the group of patients showed decreased blast transformation to PPD, preserved proliferative responses to other recall antigens, and a low PPD-induced generation of IL-2. Furthermore, as possible evidence of preactivated T cells, these patients demonstrated high soluble IL-2 receptor serum levels. Early compromise of specific cell-mediated immunity, including IL-2 abnormalities, may be of significance in newly diagnosed pulmonary TB. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
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