Calculation of Biological Variation Components and Reference Change Value for Serum Copper and Zinc
| Autor: | Müjgan Ercan, Taner Ozgurtas, Mesude Falay, Çiğdem Yücel, Turan Turhan, Erdim Sertoglu, Emel Çağlar, Ahmet Esendemir |
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| Rok vydání: | 2019 |
| Předmět: |
Adult
Male 030213 general clinical medicine chemistry.chemical_element Serum copper Zinc General Biochemistry Genetics and Molecular Biology law.invention Cohort Studies 03 medical and health sciences 0302 clinical medicine Animal science Reference Values law Biological variation Healthy volunteers Humans Blood Specimen Collection Spectrophotometry Atomic Anova test Middle Aged Copper Healthy Volunteers chemistry Reference values Female Atomic absorption spectroscopy |
| Zdroj: | Clinical Laboratory. 65 |
| ISSN: | 1433-6510 |
| DOI: | 10.7754/clin.lab.2018.181022 |
| Popis: | Background Zinc and copper are among the most important trace elements. Deficiencies of these trace elements cause a wide variety of disorders. The present study aims to report the definitive assessment of biological variation (BV) parameters for these elements as within-subject BV (CVI), between subject BV (CVG), index of individuality (II), and reference change value (RCV) in a Turkish cohort study group. Methods Ten blood specimens were collected weekly from 20 healthy volunteers (13 women, 7 men) for 10 weeks. Collected sera were stored at -80°C until the time of analysis. Serum zinc and copper levels were analyzed with atomic absorption spectrometry and ANOVA test was used to calculate the variations. Results The CVI and CVG for zinc were 6.26% and 23.27%, respectively. Analytical variation (CVA) was calculated as 4.24%. II and RCV for zinc were calculated as 0.26 and 21.51%, respectively. The CVI and CVG for copper were 6.05% and 19.64%, respectively. CVA was calculated as 4.24%. II and RCV for copper were calculated as 0.31 and 20.47%, respectively. Conclusions Since II values were less than 0.6 for both analytes, the reference values will be of little use. RCV might be preferred for better evaluation instead. |
| Databáze: | OpenAIRE |
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