The Drosophila Eip78c Gene Is Not Vital but Has a Role in Regulating Chromosome Puffs
| Autor: | Michael Ashburner, Catherine M. Goddard, Gertrud Heimbeck, Steven Russell, Adelaide T. C. Carpenter |
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| Jazyk: | angličtina |
| Rok vydání: | 1996 |
| Předmět: |
Genetics
Mutant Molecular Sequence Data Chromosome Mutagenesis (molecular biology technique) Chromosome Mapping Gene Expression Receptors Cytoplasmic and Nuclear Genes Insect Biology Investigations biology.organism_classification Chromosomes Drosophila melanogaster Nuclear receptor Mutagenesis Complementary DNA Insect Hormones Gene expression Animals Amino Acid Sequence Gene |
| Popis: | We have generated a number of chromosomal aberrations that disrupt the early-late ecdysone-induced 78C puff gene (Eip78C, ecdysone-induced protein, FlyBase name for the E78 gene of Stone and Thummel 1993), which encodes the two members of the nuclear hormone receptor superfamily Eip78C-A and Eip78C-B. The aberrations include deletions of the ligand-binding/dimeriization domain of both, inversions that split Eip78C-A but retain residual Eip78C-B expression, and a small deletion specific for Eip78C-B. We find that wild-type Eip78C functions are completely dispensable for normal development under laboratory conditions. However, we show that Eip78C-B is required for the maximal puffing activity of a subset of late puffs (63E and 82F) since these puffs are reduced in size in Eip78C-B mutant backgrounds. Paradoxically the same late puffs are reduced, as well as at least one other, when the Eip78C-B cDNA is overexpressed from a heat shock promoter. These data indicate either that Eip78C function is redundant or that it plays a subtle modulating role in the regulation of chromosome puffing. |
| Databáze: | OpenAIRE |
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