Effect of Hepatocyte Growth Factor-Transfected Human Umbilical Cord Mesenchymal Stem Cells on Hepatic Stellate Cells by Regulating Transforming Growth Factor-β1/Smads Signaling Pathway
| Autor: | Wen-Hua Jiang, Fei Yin, Li-Cui Mao, Qi-Qi Cai |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Liver Cirrhosis
Liver fibrosis Smad Proteins Biology Mesenchymal Stem Cell Transplantation Umbilical cord Umbilical Cord Transforming Growth Factor beta1 medicine Hepatic Stellate Cells Humans Cell Proliferation Hepatocyte Growth Factor Mesenchymal stem cell Mesenchymal Stem Cells Cell Biology Hematology Transfection Cell biology medicine.anatomical_structure Hepatic stellate cell Hepatocyte growth factor Signal transduction Developmental Biology medicine.drug Transforming growth factor Signal Transduction |
| Zdroj: | Stem cells and development. 30(21) |
| ISSN: | 1557-8534 |
| Popis: | Studies have shown that human umbilical cord mesenchymal stem cells (hUCMSCs) could ameliorate liver fibrosis (LF) through inhibiting the activation of hepatic stellate cells (HSCs). However, the specific mechanisms have not been studied clearly. The purpose of this study was to explore the possible mechanism of hepatocyte growth factor (HGF)-transfected hUCMSCs in inhibiting the proliferation and activation of HSCs-T6. The upper and lower double-cell coculture system was established among HGF-hUCMSCs, LV5-NC-hUCMSCs, hUCMSCs, and HSCs-T6 in experimental groups; HSCs-T6 were cultured alone as control group. After coculturing for 1, 2, and 3 days, results showed that HGF-transfected hUCMSCs could decrease cell viability of HSCs-T6 and promote apoptosis; inhibit their activation and reduce the expression of Collagen I, Collagen III, TGF-β1, Smad2 and Smad3, which may be related to inhibiting the activation of TGF-β1/Smads signaling pathway. These findings suggested that HGF-transfected hUCMSCs may be used as an alternative and novel therapeutic approach for the treatment of LF. |
| Databáze: | OpenAIRE |
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