Type III secretion homologs are present in Brucella melitensis, B. ovis, and B. suis biovars 1, 2, and 3
| Autor: | Steve Spencer, Nicola J. Commander, Abdullah I. Abdallah, Martin J. Woodward, C. Anthony Hart, Craig Winstanley |
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| Rok vydání: | 2003 |
| Předmět: |
Brucella ovis
biology Base Sequence Sequence Homology Amino Acid Brucella suis Reverse Transcriptase Polymerase Chain Reaction Biovar Brucellaceae General Medicine Brucella bacterial infections and mycoses biology.organism_classification Applied Microbiology and Biotechnology Microbiology Type three secretion system Open Reading Frames Bacterial Proteins Brucella melitensis Gene DNA Primers |
| Zdroj: | Current microbiology. 46(4) |
| ISSN: | 0343-8651 |
| Popis: | Protein sequences from characterized type III secretion (TTS) systems were used as probes in silico to identify several TTS gene homologs in the genome sequence of Brucella suis biovar 1 strain 1330. Four of the genes, named flhB, fliP, fliR, and fliF on the basis of greatest homologies to known flagellar apparatus proteins, were targeted in PCR and hybridization assays to determine their distribution among other Brucella nomen species and biovars. The results indicated that flhB, fliP, fliR and fliF are present in Brucella melitensis, Brucella ovis, and Brucella suis biovars 1, 2 and 3. Similar homologos have been reported previously in Brucella abortus. Using RT-PCR assays, we were unable to detect any expression of these genes. It is not yet known whether the genes are the cryptic remnants of a flagellar system or are actively involved in a process contributing to pathogenicity or previously undetected motility, but they are distributed widely in Brucella and merit further study to determine their role. |
| Databáze: | OpenAIRE |
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