Towards hybrid one-pot/one-electrode Pd-NPs-based nanoreactors for modular biocatalysis
| Autor: | E. V. Zolotukhina, Marcus Koch, N. Apushkinskaya, Yuliya E. Silina |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: |
Analyte
Environmental Engineering Materials science business.industry Biomedical Engineering Bioengineering Nanotechnology Small molecular weight bioanalytes Nanoreactor Modular design Electrochemistry Amperometry Multiplexed analysis Read-out mode Biocatalysis Electrode One-pot/one-electrode nanobiosensor Multiplex business Biotechnology |
| DOI: | 10.22028/d291-36743 |
| Popis: | Here, fundamental aspects affecting template-assisted engineering of oxidase-associated peroxide oxidation co-catalysis of the modeled microanalytical system based on the hybrid palladium nanoparticles (Pd-NPs) with tailored functional properties were studied. By an accurate tuning and validation of the experimental setup, a modular Pd-NPs-doped one-pot/one-electrode amperometric nanobiosensor for advanced multiplex analyte detection was constructed. The specific operational conditions (electrochemical read-out mode, pH, regeneration procedure) of the modular one-pot/one-electrode nanobiosensor allowed a reliable sensing of L -lactate (with linear dynamic range, LDR = 500 µM – 2 mM, R2 = 0.977), D -glucose (with LDR = 200 µM – 50 mM, R2 = 0.987), hydrogen peroxide (with LDR = 20 µM – 100 mM, R2 = 0.998) and glutaraldehyde (with LDR = 1 – 100 mM, R2 = 0.971). In addition, mechanistic aspects influencing the performance of Pd-NPs-doped one-pot/one-electrode for multiplex analyte sensing were studied in detail. The designed one-pot/one-electrode amperometric nanobiosensor showed a thin layer electrochemical behavior that greatly enhanced electron transfer between the functional hybrid layer and the electrode. Finally, a specific regeneration procedure of the hybrid one-pot/one-electrode and algorithm towards its usage for modular biocatalysis were developed. The reported strategy can readily be considered as a guideline towards the fabrication of commercialized nanobiosensors with tailored properties for advanced modular biocatalysis. |
| Databáze: | OpenAIRE |
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