Immobilization-Free Electrochemical Sensor Coupled with a Graphene-Oxide-Based Aptasensor for Glycated Albumin Detection

Autor: Thitirat Putnin, Wireeya Chawjiraphan, Deanpen Japrung, Nuankanya Sathirapongsasuti, Wassa Waiwinya, Dechnarong Pimalai
Rok vydání: 2021
Předmět:
Glycation End Products
Advanced
Materials science
immobilization-free electrochemical measurement
Calibration curve
lcsh:Biotechnology
Clinical Biochemistry
Oxide
Biosensing Techniques
02 engineering and technology
Electrochemistry
01 natural sciences
Article
law.invention
chemistry.chemical_compound
Glycated albumin
Limit of Detection
law
lcsh:TP248.13-248.65
glycated human serum albumin
medicine
Humans
Glycated Serum Albumin
screen-printed carbon electrode
Serum Albumin
Detection limit
Chromatography
Graphene
010401 analytical chemistry
Oxides
Electrochemical Techniques
General Medicine
Aptamers
Nucleotide
021001 nanoscience & nanotechnology
Human serum albumin
0104 chemical sciences
Electrochemical gas sensor
chemistry
diabetes mellitus
graphene oxide
Graphite
0210 nano-technology
medicine.drug
Zdroj: Biosensors
Volume 11
Issue 3
Biosensors, Vol 11, Iss 85, p 85 (2021)
ISSN: 2079-6374
Popis: An immobilization-free electrochemical sensor coupled with a graphene oxide (GO)-based aptasensor was developed for glycated human serum albumin (GHSA) detection. The concentration of GHSA was monitored by measuring the electrochemical response of free GO and aptamer-bound GO in the presence of glycated albumin
their currents served as the analytical signals. The electrochemical aptasensor exhibited good performance with a base-10 logarithmic scale. The calibration curve was achieved in the range of 0.01–50 µg/mL. The limit of detection (LOD) was 8.70 ng/mL. The developed method was considered a one-drop measurement process because a fabrication step and the probe-immobilization process were not required. This simple sensor offers a cost-effective, rapid, and sensitive detection method, and could be an alternative approach for determination of GHSA levels.
Databáze: OpenAIRE
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