In Vivo Diffusion Tensor Imaging and Histopathology of the Fimbria-Fornix in Temporal Lobe Epilepsy
| Autor: | B. M. Wheatley, Luis Concha, Christian Beaulieu, Donald W. Gross, D. J. Livy |
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| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Statistics as Topic Fornix Brain Biology behavioral disciplines and activities Temporal lobe Epilepsy Myelin In vivo medicine Image Processing Computer-Assisted Humans Brain Mapping Sclerosis General Neuroscience Histology Anatomy Articles Middle Aged medicine.disease Temporal Lobe Microscopy Electron medicine.anatomical_structure Diffusion Magnetic Resonance Imaging nervous system Epilepsy Temporal Lobe Fimbria fornix Histopathology Female psychological phenomena and processes Diffusion MRI |
| Popis: | While diffusion tensor imaging (DTI) has been extensively used to infer micro-structural characteristics of cerebral white matter in human conditions, correlations between humanin vivoDTI and histology have not been performed. Temporal lobe epilepsy (TLE) patients with mesial temporal sclerosis (MTS) have abnormal DTI parameters of the fimbria-fornix (relative to TLE patients without MTS) which are presumed to represent differences in axonal/myelin integrity. Medically intractable TLE patients who undergo temporal lobe resection including the fimbria-fornix provide a unique opportunity to study the anatomical correlates of water diffusion abnormalities in freshly excised tissue. Eleven patients with medically intractable TLE were recruited (six with and five without MTS) for presurgical DTI followed by surgical excision of a small specimen of the fimbria-fornix which was processed for electron microscopy. Blinded quantitative analysis of the microphotographs included axonal diameter, density and area, cumulative axon membrane circumference, and myelin thickness and area. As predicted by DTI the fimbria-fornix of TLE patients with MTS had increased extra-axonal fraction, and reduced cumulative axonal membrane circumference and myelin area. Consistent with the animal literature, water diffusion anisotropy over the crus of the fimbria-fornix was strongly correlated with axonal membranes (cumulative membrane circumference) within the surgical specimen (∼15% of what was analyzed with DTI). The demonstration of a correlation between histology and humanin vivoDTI, in combination with the observation thatin vivoDTI accurately predicted white matter abnormalities in a human disease condition, provides strong validation of the application of DTI as a noninvasive marker of white matter pathology. |
| Databáze: | OpenAIRE |
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