Analysis of an Engineered Salmonella Flagellar Fusion Protein, FliR-FlhB
| Autor: | Robert M. Macnab, May Kihara, Jonathan L. McMurry, John S. Van Arnam |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Salmonella
biology Blotting Western Genetic Complementation Test Membrane Proteins Flagellum biology.organism_classification medicine.disease_cause Microbiology Enterobacteriaceae Molecular biology Fusion protein Microbial Cell Biology Blot Fusion gene Bacterial Proteins Membrane protein Flagella medicine Basal body Molecular Biology |
| Zdroj: | Journal of Bacteriology. 186:2495-2498 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.186.8.2495-2498.2004 |
| Popis: | Salmonella FliR and FlhB are membrane proteins necessary for flagellar export. In Clostridium a fliR-flhB fusion gene exists. We constructed a similar Salmonella fusion gene which is able to complement fliR , flhB , and fliR flhB null strains. Western blotting revealed that the FliR-FlhB fusion protein retains the FlhB protein's cleavage properties. We conclude that the FliR and FlhB proteins are physically associated in the wild-type Salmonella basal body, probably in a 1:1 ratio. |
| Databáze: | OpenAIRE |
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