Uptake of apoptotic leukocytes by synovial lining macrophages inhibits immune complex-mediated arthritis
| Autor: | H.B.P.M. Dijkman, W.B. van den Berg, A.E.M. Holthuysen, Jhm Berden, P.L.E.M. van Lent, R. Licht |
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| Rok vydání: | 2001 |
| Předmět: |
Immunologische ontstekingsprocessen in de nier
Chemokine Phagocytosis Immunology Pathofysiologie immunologie en behandeling van nieraandoeningen Arthritis Inflammation Pathogenesis and treatment [Chronic arthritis] Inflammatory reactions in the kidneys medicine Immunology and Allergy biology business.industry Pathogenese en behandeling [Chronische arthritis] Chemotaxis Pathophysiology immunology and treatment of renal disease Cell Biology medicine.disease Immune complex Cell biology medicine.anatomical_structure biology.protein medicine.symptom Synovial membrane business Immune complex disease |
| Zdroj: | Journal of Leukocyte Biology, 70, 5, pp. 708--14 Journal of Leukocyte Biology, 70, 708--14 |
| ISSN: | 0741-5400 |
| Popis: | Previously we have shown that synovial lining macrophages (SLMs) determine the onset of experimental immune complex–mediated arthritis (ICA). During joint inflammation, many leukocytes undergo apoptosis, and removal of leukocytes by SLMs may regulate resolution of inflammation. In this study we investigated binding and uptake of apoptotic leukocytes by SLMs and its impact on the onset of murine experimental arthritis. We used an in vitro model to evaluate phagocytosis of apoptotic cells on chemotaxis. Phagocytosis of apoptotic thymocytes resulted in a significant decrease (58%) of chemotactic activity for polymorphonuclear neutrophils (PMNs). If apoptotic cells were injected directly into a normal murine knee joint, SLMs resulted in a prominent uptake of cells. After ICA induction, electron micrographs showed that apoptotic leukocytes were evidently present in SLMs on days 1 and 2. Injection of apoptotic leukocytes into the knee joint 1 h before induction of ICA significantly inhibited PMN infiltration into the knee joint at 24 h (61% decrease). This study indicates that uptake of apoptotic leukocytes by SLM reduces chemotactic activity and inhibits the onset of experimental arthritis. These findings indicate an important mechanism in the resolution of joint inflammation. |
| Databáze: | OpenAIRE |
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