Cloning and functional expression of a brain G-protein-coupled ATP receptor

Autor:	Joseph Simon, Eric A. Barnard, Brian F. King, Alan N. Bateson, Belinda J. Krishek, Tania E. Webb, Geoffrey Burnstock, Trevor G. Smart
Rok vydání:	1993
Předmět:	G-protein-coupled receptor Transcription Genetic G protein Xenopus Molecular Sequence Data Biophysics Chick Embryo Biology Biochemistry P2Y subtype Adenosine Triphosphate Structural Biology Transcription (biology) GTP-Binding Proteins Complementary DNA Genetics Animals Tissue Distribution Amino Acid Sequence RNA Messenger P2 purinoceptor Cloning Molecular Molecular Biology Peptide sequence G protein-coupled receptor chemistry.chemical_classification Brain Chemistry Base Sequence Sequence Homology Amino Acid cDNA library Electric Conductivity Receptors Purinergic RNA Cell Biology Molecular biology Recombinant Proteins Cell biology Amino acid ATP chemistry Oocytes Chicken brain
Zdroj:	FEBS letters. 324(2)
ISSN:	0014-5793
Popis:	A cDNA encoding a novel member of the G-protein-coupled receptor (GCR) superfamily, an ATP receptor, has been isolated from an embryonic chick whole brain cDNA library by hybridization screening. The encoded protein has a sequence of 362 amino acids (41 kDa) and shares no more than 27% amino acid identity with any known GCR. When expressed as a complementary RNA (cRNA) in Xenopus oocytes a slowly-developing inward current was observed in response to application of ATP. The pharmacology of this expressed protein defines it as a P2Y purinoceptor.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1bb1c8227f2e70d20e43246568ef1bdb https://pubmed.ncbi.nlm.nih.gov/8508924 Zobrazit plný text záznamu Plný text