Retrospective Proteomic Screening of 100 Breast Cancer Tissues
Autor: | Nadia Ninfa Albanese, Rosa Musso, Salvatore Minafra, Patrizia Cancemi, Elena Roz, Gianluca Di Cara, Ida Pucci-Minafra |
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Přispěvatelé: | Pucci, I., Di Cara, G., Musso, R., Cancemi, P., Albanese, N., Roz, E., Minafra, S. |
Rok vydání: | 2017 |
Předmět: |
0301 basic medicine
Gene isoform Clinical Biochemistry gel-based proteomic lcsh:QR1-502 Motility surgical tissue gel-based proteomics Biology Bioinformatics Proteomics Biochemistry lcsh:Microbiology Article Metastasis 03 medical and health sciences Breast cancer breast cancer Structural Biology Medicine Settore BIO/06 - Anatomia Comparata E Citologia Molecular Biology oncology_oncogenics mass spectrometry surgical tissues business.industry Cancer medicine.disease Primary tumor 030104 developmental biology Apoptosis protein clustering Cancer research business |
Zdroj: | Proteomes Proteomes; Volume 5; Issue 3; Pages: 15 Proteomes, Vol 5, Iss 3, p 15 (2017) |
ISSN: | 2227-7382 |
Popis: | The present investigation has been conducted on one hundred tissue fragments of breast cancer, collected and immediately cryopreserved following the surgical resection. The specimens were selected from patients with invasive ductal carcinoma of the breast, the most frequent and potentially aggressive type of mammary cancer, with the objective to increase the knowledge of breast cancer molecular markers potentially useful for clinical applications. The proteomic screening; by 2D-IPG and mass spectrometry; allowed us to identify two main classes of protein clusters: proteins expressed ubiquitously at high levels in all patients; and proteins expressed sporadically among the same patients. Within the group of ubiquitous proteins, glycolytic enzymes and proteins with anti-apoptotic activity were predominant. Among the sporadic ones, proteins involved in cell motility, molecular chaperones and proteins involved in the detoxification appeared prevalent. The data of the present study indicates that the primary tumor growth is reasonably supported by concurrent events: the inhibition of apoptosis and stimulation of cellular proliferation, and the increased expression of glycolytic enzymes with multiple functions. The second phase of the evolution of the tumor can be prematurely scheduled by the occasional presence of proteins involved in cell motility and in the defenses of the oxidative stress. We suggest that this approach on large-scale 2D-IPG proteomics of breast cancer is currently a valid tool that offers the opportunity to evaluate on the same assay the presence and recurrence of individual proteins, their isoforms and short forms, to be proposed as prognostic indicators and susceptibility to metastasis in patients operated on for invasive ductal carcinoma of the breast. |
Databáze: | OpenAIRE |
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