Cholesterol loaded cyclodextrin increases freezability of buffalo bull (Bubalus bubalis) spermatozoa by increasing cholesterol to phospholipid ratio
| Autor: | Megha Pande, Srikant Ghosh, S.S. Ramteke, N. C. Barik, G. K. Das, J. K. Prasad, J.S. Rajoriya |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
chemistry.chemical_classification
buffalo General Veterinary biology Phospholipid ratio Cyclodextrin Cholesterol urogenital system cholesterol:phospholipid (C/P) ratio Veterinary medicine Semen biology.organism_classification SF1-1100 Animal culture Andrology chemistry.chemical_compound chemistry cholesterol loaded cyclodextrin SF600-1100 Bubalus |
| Zdroj: | Veterinary World, Vol 7, Iss 9, Pp 702-706 (2014) |
| ISSN: | 2231-0916 0972-8988 |
| Popis: | Aim: The study was conducted to investigate the effect of cholesterol loaded cyclodextrin (CLC) on freezability of buffalo spermatozoa. Materials and Methods: Murrah buffalo bull semen samples with progressive motility of 70% and greater were used. After the evaluation of motility and livability, four equal fractions of semen samples were made. Group I was kept as control and diluted with Tris, whereas Group II, III and IV were treated with CLC solution at the rate of 2.0, 3.0 and 4.0 mg/ml respectively to obtain 120 × 106 sperm/ml as final spermatozoa concentration. The aliquots of all the groups were incubated for action of CLC, followed by dilution and freezing. Evaluation at pre-freeze and post-thaw stage of progressive motility, viability and level of cholesterol and phospholipid was done. Results: The mean cholesterol content (μg/100 × 106 spermatozoa) of Group I, II, III and IV at pre-freeze stage was 21.55±0.63, 49.56±1.38, 55.67±0.45 and 47.79±1.01 and at post-thaw stage were 13.18±0.45, 34.27±0.71, 36.21±0.48 and 33.68±0.56, respectively. At pre-freeze stage, cholesterol content was significantly (p |
| Databáze: | OpenAIRE |
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