Effects of 4-nonylphenol on hepatic gene expression of peroxisome proliferator-activated receptors and cytochrome P450 isoforms (CYP1A1 and CYP3A4) in juvenile sole (Solea solea)
| Autor: | Francesco Alessandro Palermo, Paolo Cocci, Gilberto Mosconi |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Gene isoform
medicine.medical_specialty Environmental Engineering Health Toxicology and Mutagenesis Peroxisome Proliferator-Activated Receptors Peroxisome proliferator-activated receptor Gene Expression Retinoid X receptor Phenols Internal medicine Gene expression medicine Cytochrome P-450 CYP1A1 Environmental Chemistry Animals Cytochrome P-450 CYP3A PPAR alpha RNA Messenger Receptor chemistry.chemical_classification biology CYP3A4 Public Health Environmental and Occupational Health Cytochrome P450 General Medicine General Chemistry Pollution Endocrinology chemistry Liver biology.protein Flatfishes Signal transduction Water Pollutants Chemical |
| Zdroj: | Chemosphere. 93(6) |
| ISSN: | 1879-1298 |
| Popis: | The objective of the present study was to investigate the modulatory effects of the xenoestrogen 4-nonylphenol (4-NP) on hepatic peroxisome proliferator-activated receptor (PPAR) α and β gene expression patterns in relation to the detoxification pathways mediated by cytochrome P450 isoforms (CYP1A1 and CYP3A4). Waterborne 4-NP-induced effects were compared with those of 10 −8 M 17β-estradiol (E2) by using in vivo dose–response experiments carried out with juvenile sole ( Solea solea ). Compared to the controls, significantly higher levels of PPARα mRNAs were found in fish treated with E2 or 4-NP (10 −6 M) 3 d after exposure; the highest dose of 4-NP also caused up-regulation of retinoid X receptor α (RXRα) transcript levels. On the contrary, PPARβ gene expression was not modulated by E2 or 4-NP. Our data show that 4-NP-induced PPARα mRNA levels coincide with suppression of CYP1A1 and CYP3A4 expression similarly to E2. The results from these in vivo studies suggest the presence of cross-talk between nuclear receptor-mediated signaling pathways and PPARα that may result in modulation of CYP450 isoforms expression following 4-NP treatment in sole liver. |
| Databáze: | OpenAIRE |
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