Disappearance of free SH-groups in hemoglobin of man, rat and rabbit after exposure to alkylating agents in vitro
| Autor: | Hennie M.J. Roelofs, P.J.L. van Gemert, P.Th. Henderson, J.M. Neis |
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| Rok vydání: | 1984 |
| Předmět: |
Male
Alkylating Agents Sh groups Alkylation In Vitro Techniques Biology Toxicology Hemoglobins chemistry.chemical_compound Styrene oxide Animals Humans Sulfhydryl Compounds Lagomorpha Rats Inbred Strains biology.organism_classification In vitro Rats Methyl methanesulfonate chemistry Biochemistry Spectrophotometry Iodoacetamide Rabbits Hemoglobin |
| Zdroj: | Toxicology. 31:319-327 |
| ISSN: | 0300-483X |
| Popis: | The effect of alkylating agents on the content of free SH-groups in human, rat and rabbit hemoglobin was examined. Treatment of blood in vitro with iodoacetamide, styrene oxide and methyl methanesulfonate resulted in a dose-dependent decrease of free SH-groups in hemoglobin. The free SH-groups of rat hemoglobin were more susceptible to the alkylating agents tested than those of human and rabbit hemoglobin. The method is based on the isolation of hemoglobin and a subsequent measurement of the free SH-content of the hemoglobin by means of a spectroptometric procedure. This procedure may be useful to examine differences in susceptibility of free SH-groups in hemoglobin, e.g. of different animal species, and on the other hand, to study the potencies of different electrophilic agents to alkylate these nucleophilic centers. |
| Databáze: | OpenAIRE |
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