HGF/SF Increases Tumor Blood Volume: A Novel Tool for the In Vivo Functional Molecular Imaging of Met
| Autor: | Dafna Kaufman, Gideon Y. Stein, Ilan Tsarfaty, Brain Cao, George F. Vande Woude, Galia Tsarfaty, Sharon Moshitch-Moshkovitz, James H. Resau |
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| Jazyk: | angličtina |
| Rok vydání: | 2006 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Receptor tyrosine kinase Blood volume Biology lcsh:RC254-282 03 medical and health sciences 0302 clinical medicine In vivo medicine contrast medium ultrasound 030304 developmental biology 0303 health sciences medicine.diagnostic_test Magnetic resonance imaging lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens In vitro 3. Good health Contrast medium 030220 oncology & carcinogenesis biology.protein Met Hepatocyte growth factor Molecular imaging hepatocyte growth factor/scatter factor functional molecular imaging medicine.drug |
| Zdroj: | Neoplasia: An International Journal for Oncology Research, Vol 8, Iss 5, Pp 344-352 (2006) |
| ISSN: | 1522-8002 1476-5586 |
| Popis: | Molecular functional and metabolic imaging allows visualization of disease-causing processes in living organisms. Here we present a new approach for the functional molecular imaging (FMI) of endogenous tyrosine kinase receptor activity using Met and its ligand, hepatocyte growth factor/scatter factor (HGF/ SF), as a model. HGF/SF and Met play significant roles in the biology and pathogenesis of a wide variety of cancers and, therefore, may serve as potential targets for cancer prognosis and therapy. We have previously shown that Met activation by HGF/SF increases oxygen consumption in vitro and results in substantial alteration of blood oxygenation levels in vivo , as measured by blood oxygenation level-dependent magnetic resonance imaging. Using contrast medium (CM) ultrasound imaging, we demonstrate here that HGF/SF induces an increase in tumor blood volume. This increase is evident in small vessels, including vessels that were not detected before HGF/SF treatment. The specificity of the effect was validated by its inhibition using anti-HGF/SF antibodies. This change in tumor hemodynamics, induced by HGF/SF and measured by CM ultrasound, is further used as a tool for Met FMI in tumors. This novel noninvasive molecular imaging technique may be applied for the in vivo diagnosis, prognosis, and therapy of Met-expressing tumors. References |
| Databáze: | OpenAIRE |
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