Ferulic Acid Orchestrates Anti-Oxidative Properties of Danggui Buxue Tang, an Ancient Herbal Decoction: Elucidation by Chemical Knock-Out Approach
| Autor: | Huai Y Wang, Karl Wah Keung Tsim, Amy G. W. Gong, Vincent Y. Huang, Huang Q. Lin, Tina T. X. Dong |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Protein Expression Glycobiology lcsh:Medicine Decoction Plant Roots Biochemistry Antioxidants Ferulic acid chemistry.chemical_compound Oxidative Damage 0302 clinical medicine lcsh:Science Active ingredient chemistry.chemical_classification Liquid Chromatography Multidisciplinary Traditional medicine biology GCLM Luciferase Assay Chromatographic Techniques Astragalus propinquus Enzymes Bioassays and Physiological Analysis 030220 oncology & carcinogenesis Oxidoreductases Luciferase Research Article Angelica sinensis Coumaric Acids DNA transcription Research and Analysis Methods Cell Line 03 medical and health sciences Polysaccharides Genetics Gene Expression and Vector Techniques Humans Molecular Biology Techniques Molecular Biology Enzyme Assays Reactive oxygen species Molecular Biology Assays and Analysis Techniques Biology and life sciences lcsh:R Proteins Phenolic acid biology.organism_classification Antioxidant Response Elements High Performance Liquid Chromatography 030104 developmental biology Enzyme chemistry Enzymology lcsh:Q Gene expression Reactive Oxygen Species Biochemical Analysis Drugs Chinese Herbal |
| Zdroj: | PLoS ONE PLoS ONE, Vol 11, Iss 11, p e0165486 (2016) |
| ISSN: | 1932-6203 |
| Popis: | Ferulic acid, a phenolic acid derived mainly from a Chinese herb Angelica Sinensis Radix (ASR), was reported to reduce the formation of free radicals. Danggui Buxue Tang (DBT), a herbal decoction composing of Astragali Radix (AR) and ASR, has been utilized for more than 800 years in China having known anti-oxidative property. Ferulic acid is a major active ingredient in DBT; however, the role of ferulic acid within the herbal mixture has not been resolved. In order to elucidate the function of ferulic acid within this herbal decoction, a ferulic acid-depleted herbal decoction was created and named as DBTΔfa. The anti-oxidative properties of chemically modified DBT decoction were systemically compared in cultured H9C2 rat cardiomyoblast cell line. The application of DBT and DBTΔfa into the cultures showed functions in (i) decreasing the reactive oxygen species (ROS) formation, detected by laser confocal; (ii) increasing of the activation of Akt; (iii) increasing the transcriptional activity of anti-oxidant response element (ARE); and (iv) increasing the expressions of anti-oxidant enzymes, i.e. NQO1 and GCLM. In all scenario, the aforementioned anti-oxidative properties of DBTΔfa in H9C2 cells were significantly reduced, as compared to authentic DBT. Thus, ferulic acid could be an indispensable chemical in DBT to orchestrate multi-components of DBT as to achieve maximal anti-oxidative functions. |
| Databáze: | OpenAIRE |
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