Upregulated miR-203a-3p and its potential molecular mechanism in breast cancer: A study based on bioinformatics analyses and a comprehensive meta-analysis

Autor:	Jin‑Che Zhao, Cai‑Xia Feng, Rong‑Quan He, Kai‑Teng Cai, Jie Ma, Jin‑Cai Zhong
Jazyk:	angličtina
Rok vydání:	2018
Předmět:	0301 basic medicine Adult Male Cancer Research Breast Neoplasms Biology medicine.disease_cause Biochemistry 03 medical and health sciences 0302 clinical medicine Breast cancer breast cancer microRNA Protein Interaction Mapping Genetics medicine Biomarkers Tumor Humans Protein Interaction Maps KEGG Molecular Biology Gene Aged Neoplasm Staging Regulation of gene expression gene expression omnibus Oncogene target gene Gene Expression Profiling Computational Biology Articles Middle Aged medicine.disease Prognosis Molecular medicine Gene Expression Regulation Neoplastic MicroRNAs 030104 developmental biology Gene Ontology Oncology the cancer genome atlas ROC Curve 030220 oncology & carcinogenesis Cancer research Molecular Medicine Female Carcinogenesis microRNA-203a-3p Transcriptome
Zdroj:	Molecular Medicine Reports
ISSN:	1791-3004 1791-2997
Popis:	Breast cancer (BC) has been identified as the leading malignancy in women worldwide. However, the potential molecular mechanism of microRNA (miR)‑203a‑3p in BC remains to be elucidated. The present study evaluated the expression of miR‑203a‑3p in BC and adjacent normal tissue in several publically available datasets. The distinguishability of precursor miR‑203a and miR‑203a‑3p in BC tissue and adjacent breast tissue was assessed using receiver operating characteristic (ROC) and summarized ROC (sROC) approaches. In addition, gene ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes pathway analysis and protein‑protein interaction analysis were performed to determine the potential molecular mechanism of miR‑203a‑3p in BC. It was identified that the expression of precursor miR‑203a was markedly upregulated in 1,077 BC tissue samples compared to 104 adjacent breast tissue samples from The Cancer Genome Atlas. Additionally, an increasing trend in miR‑203a‑3p expression was observed in 756 BC tissue samples compared with 76 adjacent breast tissue samples from the University of California Santa Cruz Xena project. In addition, a comprehensive meta‑analysis suggested that the expression of miR‑203a‑3p was markedly increased in 2,444 BC tissue samples compared with 559 adjacent breast tissue samples. The area under the curve of the ROC and sROC revealed that miR‑203a‑3p expression was able to distinguish between BC tissue and adjacent breast tissue. However, miR‑203a‑3p exhibited no prognostic value in BC. The results of GO enrichment demonstrated that the miR‑203a target genes were associated with 'plasma membrane integrity', 'cell surface receptor linked signal and transduction' and '3',5'‑cyclic nucleotide phosphodiesterase activity'. 'Purine metabolism' was identified as the pathway with the most enrichment of miR‑203a‑3p target genes in BC. The present study also identified insulin‑like growth factor receptor (IGF1) as a hub gene associated with miR‑203a in BC. In summary, miR‑203a‑3p may enhance the development and oncogenesis of BC, and IGF1 was defined as a hub gene of miR‑203a‑3p in BC.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1ba7f8de3d76989efa49dc5f4e9cae45 http://europepmc.org/articles/PMC6236224 Zobrazit plný text záznamu Plný text ve formátu PDF Plný text ve formátu HTML
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