Integrated Microfluidic Compact Disc Device with Potential Use in Both Centralized and Point-of-Care Laboratory Settings
| Autor: | Ann-Kristin Honerud, Mats Inganäs, Ann Eckersten, Gunnar Ekstrand, Helene Derand, Per Andersson, Gunnar Thorsén, Tobias Söderman, Gérald Jesson |
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| Rok vydání: | 2005 |
| Předmět: |
chemistry.chemical_classification
Streptavidin Materials science Myoglobin Capillary action Point-of-Care Systems Microfluidics Biochemistry (medical) Clinical Biochemistry Compact disc Nanotechnology Polymer Microfluidic Analytical Techniques Sensitivity and Specificity chemistry.chemical_compound chemistry Reagent Humans Interleukin-2 Metering mode Laboratories Interleukin-1 Point of care |
| Zdroj: | Clinical Chemistry. 51:1985-1987 |
| ISSN: | 1530-8561 0009-9147 |
| Popis: | Reducing the size of reaction volumes in immunodiagnostic procedures can reduce not only reagent cost but also analysis time. With reduced sample and reagent volumes and use of a flow-through mode, reactions reach completion rapidly, potentially decreasing turnaround time and making such systems suitable for point-of-care testing. However, decreased reaction volumes typically lead to other problems, such as imprecision of liquid metering, liquid evaporation, and problems related to the increased surface-to-volume ratio. To address these challenges, we have developed a new bioanalytical system composed of a disposable microfluidic compact disc (CD) device (Gyrolab Bioaffy™) that is intended for sandwich immunoassays and an instrument for automatic processing of CDs (Gyrolab™ Workstation). The Gyrolab Bioaffy CD (diameter, 120 mm) contains 104 parallel microstructures conveniently fitting into the CD, each covering a surface area of ∼2 × 15 mm. The CD is manufactured by injection molding using a cycloolefin polymer. The CD is covered with a lid in which appropriately placed holes enable liquid introduction by the robot into the microstructures; the lid protects liquids from evaporation once in the CD. Liquid movement and localization are achieved by a combination of capillary action, centrifugal force, and hydrophobic barriers within the microstructure. Each microstructure contains a 15-nL particle-based column coupled with streptavidin in which reactions take place (Fig. 1⇓ ). The Gyrolab Bioaffy is designed to be an open system; i.e., it is compatible with various assays that use biotinylated reagents, which bind to the streptavidin column … |
| Databáze: | OpenAIRE |
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