Enantiomeric separation of dansyl amino acids using macrocyclic antibiotics as chiral mobile phase additives by narrow-bore high-performance liquid chromatography
| Autor: | V. Scott Sharp, Maureen N. Letts, John P. Rose, Donald S. Risley |
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| Rok vydání: | 2004 |
| Předmět: |
inorganic chemicals
Pharmacology chemistry.chemical_classification Analyte Chromatography Chemistry organic chemicals Organic Chemistry Enantioselective synthesis High-performance liquid chromatography Catalysis Capacity factor Analytical Chemistry Amino acid Drug Discovery polycyclic compounds heterocyclic compounds Enantiomer Selectivity Chiral derivatizing agent Spectroscopy |
| Zdroj: | Chirality. 16:153-161 |
| ISSN: | 1520-636X 0899-0042 |
| DOI: | 10.1002/chir.20002 |
| Popis: | Seven macrocyclic antibiotics were evaluated as chiral selectors for the enantiomeric separation of 11 dansyl amino acids using narrow-bore high-performance liquid chromatography (HPLC). The macrocyclic antibiotics were incorporated as mobile phase additives to determine the enantioselective effects on the chiral analytes. The resolution and capacity factor (k') of each analyte were assessed while varying the structure of macrocyclic antibiotic and the mobile phase buffer pH. The selectivity of the chiral selectors was measured as a function of changes in these parameters. All 11 dansyl amino acids were separated by at least one of the chiral selectors. Three-dimensional computer modeling of the more effective chiral selectors illustrated the importance of macrocyclic antibiotic structure concerning stereospecific analyte interaction. |
| Databáze: | OpenAIRE |
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