Molecular level investigation of the role of peptide interactions in the glyphosate analytics
| Autor: | Peter Gros, Ashour A. Ahmed, Oliver Kühn, Peter Leinweber |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
endocrine system
Environmental Engineering Ultraviolet Rays Health Toxicology and Mutagenesis Glycine Peptide 010501 environmental sciences 01 natural sciences Chloride chemistry.chemical_compound medicine Environmental Chemistry Molecule Derivatization 0105 earth and related environmental sciences chemistry.chemical_classification Fluorenes Chromatography Herbicides digestive oral and skin physiology 010401 analytical chemistry Public Health Environmental and Occupational Health Albumin General Medicine General Chemistry Chromophore Pollution 0104 chemical sciences Models Chemical chemistry Glyphosate Peptides hormones hormone substitutes and hormone antagonists medicine.drug |
| Zdroj: | Chemosphere. 196:129-134 |
| ISSN: | 0045-6535 |
| DOI: | 10.1016/j.chemosphere.2017.12.162 |
| Popis: | The detection of the herbicide glyphosate (GLP) in environmental samples is most often conducted after derivatizing the target molecule with the chromophore 9-fluorenylmethyloxycarbonyl chloride (FMOC-Cl). However, this method is sensitive to all primary and secondary amines, which can occur in the sample matrix as well. In order to quantify the interference of primary and secondary amines on GLP detection, we have used well-defined peptides such as pentaglycine (PG) and albumin as well as mixtures of peptides such as peptone. These peptides have been added to the derivatization solution of GLP at different constant concentration levels and UV extinction coefficients have been determined. Data analysis supported by quantum chemical modeling of the GLP-peptide, FMOC-GLP, and FMOC-peptide complexation reactions facilitated the identification of two interfering impacts of peptide on GLP derivatization: (i) increase of the signal due to reaction with FMOC-Cl leading to an overestimation of GLP concentration and (ii) decrease of GLP recovery due to complex formation and therefore inhibition of GLP derivatization, which leads to an underestimation. Specifically, our results indicated that the GLP-peptide- and peptide-FMOC-interactions are mainly affected by type of interfering peptides as well as concentration of each peptide and GLP in the environmental samples. |
| Databáze: | OpenAIRE |
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