The influence of a single hemodialysis procedure on human T lymphocytes
| Autor: | Hanna Storoniak, Izabella Kuźmiuk-Glembin, Krzysztof Pietruczuk, Jacek M. Witkowski, Alicja Dębska-Ślizień, Małgorzata Pindel, Katarzyna A. Lisowska |
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| Rok vydání: | 2019 |
| Předmět: |
CD4-Positive T-Lymphocytes
Male 0301 basic medicine medicine.medical_specialty CD4-CD8 Ratio lcsh:Medicine CD8-Positive T-Lymphocytes Article Flow cytometry Immunomodulation 03 medical and health sciences 0302 clinical medicine Immune system Renal Dialysis Internal medicine medicine Humans lcsh:Science Aged Cell Proliferation Aged 80 and over Multidisciplinary medicine.diagnostic_test Chemistry lcsh:R T lymphocyte Middle Aged medicine.disease Acquired immune system Uremia 030104 developmental biology Endocrinology Cytokines Female lcsh:Q Tumor necrosis factor alpha 030217 neurology & neurosurgery Ex vivo CD8 |
| Zdroj: | Scientific Reports, Vol 9, Iss 1, Pp 1-9 (2019) Scientific Reports |
| ISSN: | 2045-2322 |
| DOI: | 10.1038/s41598-019-41619-x |
| Popis: | At the moment it is unknown to what extent the impaired function of T lymphocytes in ESRD patients depends on uremia, and to what extent on hemodialysis (HD) procedure. Therefore, the purpose of the study was to evaluate percentages of T lymphocyte subpopulations ex vivo, plasma concentrations of IL12p70, TNF, IL-10, IL-6, IL-1β, IL-8 cytokines and selected proliferation parameters of in vitro activated T lymphocytes in HD patients before and after single HD procedure using flow cytometry. We demonstrated that the percentage of CD8+ cells ex vivo was decreased while the CD4+/CD8+ ratio was increased after HD procedure. Also, there was significant decrease in the percentage of CD8+HLA-DR+, CD8+CD69+ and CD8+CD95+ cells after HD. At the same time, an increase in the percentage of CD4+CD95+ cells was observed after HD. From all analyzed cytokines, only the concentration of IL-8 was significantly decreased after HD procedure. A single HD session enhanced proliferation capacity of CD4+ cells but not CD8+ cells in vitro by increasing number of cell divisions and percentage of dividing cells. Our results show that a single hemodialysis can have immunomodulatory effect on HD patients and may contribute to the state of immune deficiency observed in patients with ESRD. |
| Databáze: | OpenAIRE |
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