DNA damage and 2′-deoxyguanosine oxidation induced by S(IV) autoxidation catalyzed by copper(II) tetraglycine complexes: Synergistic effect of a second metal ion
| Autor: | Nina Coichev, María V. Alipázaga, Edlaine Linares, Ruben G. M. Moreno, Osmar F. Gomes, Marisa Helena Gennari de Medeiros |
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| Rok vydání: | 2007 |
| Předmět: |
DNA damage
Metal ions in aqueous solution Radical chemistry.chemical_element Photochemistry Biochemistry Medicinal chemistry Catalysis Inorganic Chemistry Metal chemistry.chemical_compound Organometallic Compounds Sulfur Dioxide Deoxyguanosine Electrophoresis Agar Gel Autoxidation Electron Spin Resonance Spectroscopy Copper chemistry Spectrophotometry visual_art visual_art.visual_art_medium Oligopeptides Oxidation-Reduction DNA Damage |
| Zdroj: | Journal of Inorganic Biochemistry. 101:866-875 |
| ISSN: | 0162-0134 |
| Popis: | S(IV) (SO(2),HSO(3)(-)andSO(3)(2-)) autoxidation catalyzed by Cu(II)/tetraglycine complexes in the presence of DNA or 2'-deoxyguanosine (dGuo) resulted in DNA strand breaks and formation of 8-oxo-7,8-dihydro-2'-deoxyguanosine (8-oxodGuo), respectively. Ni(II), Co(II) or Mn(II) (1.0x10(-4)M) complexes had much smaller effects. Cu(II)/tetraglycine (1.0x10(-4)M) in the presence of Ni(II) or Mn(II) (10(-7)-10(-6)M) and S(IV) showed remarkable synergistic effect with these metal ions producing a higher yield of 8-oxodGuo. Oxidation of dGuo and DNA damage were attributed to oxysulfur radicals formed as intermediates in S(IV) autoxidation catalyzed by transition metal ions. SO*(3)(-) and HO* radicals were detected by EPR-spin trapping experiments with DMPO (5,5-dimethyl-1-pyrroline-N-oxide). |
| Databáze: | OpenAIRE |
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