Wnt6 activates endoderm in the sea urchin gene regulatory network

Autor: David R. McClay, Esther Miranda, Thierry Lepage, Jenifer C. Croce, Jeff Chieh-fu Peng, Guy Lhomond, Ryan C. Range, Shu-Yu Wu
Přispěvatelé: Laboratoire de Biologie du Développement de Villefranche sur mer (LBDV), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut de la Mer de Villefranche (IMEV), Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS), Sorbonne Université (SU), Auburn University (AU), Colorado School of Mines, Departament de Matemàtiques [Barcelona] (UAB), Universitat Autònoma de Barcelona (UAB), Institut de Biologie Valrose (IBV), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Duke University [Durham], Croce, Jenifer, Université Nice Sophia Antipolis (1965 - 2019) (UNS)
Rok vydání: 2011
Předmět:
MESH: Signal Transduction
Embryo
Nonmammalian
MESH: Embryonic Induction
WNT6
0302 clinical medicine
MESH: Gene Expression Regulation
Developmental
MESH: Animals
Gene Regulatory Networks
[SDV.BDD]Life Sciences [q-bio]/Development Biology
Sea urchin
Research Articles
MESH: Gene Regulatory Networks
Embryonic Induction
Genetics
chemistry.chemical_classification
0303 health sciences
biology
Endoderm
Wnt signaling pathway
Gene Expression Regulation
Developmental
Embryo
MESH: Endoderm
Cell biology
Dishevelled
MESH: Wnt Proteins
medicine.anatomical_structure
Gene Knockdown Techniques
embryonic structures
Signal Transduction
Mesoderm
animal structures
MESH: Oocytes
03 medical and health sciences
Endomesoderm
biology.animal
[SDV.BDD] Life Sciences [q-bio]/Development Biology
medicine
Animals
Molecular Biology
030304 developmental biology
MESH: Embryo
Nonmammalian
MESH: Gene Knockdown Techniques
MESH: Sea Urchins
Wnt Proteins
chemistry
Sea Urchins
Oocytes
030217 neurology & neurosurgery
Developmental Biology
Zdroj: Development (Cambridge, England)
Development (Cambridge, England), Company of Biologists, 2011, 138 (15), pp.3297-3306. ⟨10.1242/dev.058792⟩
Development (Cambridge, England), 2011, 138 (15), pp.3297-3306. ⟨10.1242/dev.058792⟩
ISSN: 1477-9129
0950-1991
DOI: 10.1242/dev.058792
Popis: International audience; In the sea urchin, entry of β-catenin into the nuclei of the vegetal cells at 4th and 5th cleavages is necessary for activation of the endomesoderm gene regulatory network. Beyond that, little is known about how the embryo uses maternal information to initiate specification. Here, experiments establish that of the three maternal Wnts in the egg, Wnt6 is necessary for activation of endodermal genes in the endomesoderm GRN. A small region of the vegetal cortex is shown to be necessary for activation of the endomesoderm GRN. If that cortical region of the egg is removed, addition of Wnt6 rescues endoderm. At a molecular level, the vegetal cortex region contains a localized concentration of Dishevelled (Dsh) protein, a transducer of the canonical Wnt pathway; however, Wnt6 mRNA is not similarly localized. Ectopic activation of the Wnt pathway, through the expression of an activated form of β-catenin, of a dominant-negative variant of GSK-3β or of Dsh itself, rescues endomesoderm specification in eggs depleted of the vegetal cortex. Knockdown experiments in whole embryos show that absence of Wnt6 produces embryos that lack endoderm, but those embryos continue to express a number of mesoderm markers. Thus, maternal Wnt6 plus a localized vegetal cortical molecule, possibly Dsh, is necessary for endoderm specification; this has been verified in two species of sea urchin. The data also show that Wnt6 is only one of what are likely to be multiple components that are necessary for activation of the entire endomesoderm gene regulatory network.
Databáze: OpenAIRE
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