Characterization of an Acidic Polysaccharides from Carrot and Its Hepatoprotective Effect on Alcoholic Liver Injury in Mice
| Autor: | Xinyue Li, Xiangying Kong, Wenjun Xu, Wei Liang, Meng Qiu, Hongman Chen |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Rhamnose
Bioengineering Polysaccharide Protective Agents Biochemistry Daucus chemistry.chemical_compound Mice Microscopy Electron Transmission Polysaccharides Lipid droplet medicine Animals Molecular Biology Liver Diseases Alcoholic Triglycerides chemistry.chemical_classification Liver injury biology Alcohol Dehydrogenase Fructose General Chemistry General Medicine Aldehyde Dehydrogenase biology.organism_classification medicine.disease Daucus carota Molecular Weight Disease Models Animal Oxidative Stress Cholesterol chemistry Liver Galactose Molecular Medicine |
| Zdroj: | Chemistrybiodiversity. 18(8) |
| ISSN: | 1612-1880 |
| Popis: | The characteristics of acidic polysaccharides extracted from Daucus carota L. var. sativa Hoffm were investigated and its hepatoprotective effects on alcoholic liver injury were determined in the mice model. A carrot polysaccharide (CPS-I: Carrot polysaccharide-I) with the molecular weight of 3.40×104 kDa was isolated from Daucus carota L. and purified by diethylaminoethyl-52 and Sephadex G-150 column chromatography. The components were analyzed by HPLC, which revealed that CPS-I consisted of galacturonic acid, rhamnose, xylose, arabinose, fructose, and galactose at a relative ratio of 1 : 3.16 : 1.13 : 5.53 : 3.45 : 7.76. Structural characterization analysis suggested that CPS-I was mainly composed of →6)-β-D-Galp-(1→ and →5)-α-L-Araf-(1→. The hepatoprotective effect of CPS-I was evaluated by alcoholic liver injury mice model. The results showed that the administration of CPS-I (300 mg/kg/day) alleviated the alcoholic liver injury in mice by increasing the levels of ADH and ALDH and reducing oxidative stress. CPS-I ameliorated the pathological changes of liver characterized by lipid accumulation, and reduced the number of lipid droplets. |
| Databáze: | OpenAIRE |
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