Evidence for internal stores of CCR5 in blood cells

Autor:	Stefano Marullo, Alain Thuret, Mark G. H. Scott, Catherine Labbé-Jullié, Lamia Achour, Hamasseh Shirvani, Georges Bismuth
Rok vydání:	2011
Předmět:	CD4-Positive T-Lymphocytes Receptors CCR5 viruses Immunology Cell virus diseases Cell Biology Hematology CD8-Positive T-Lymphocytes Biology Biochemistry Cell biology medicine.anatomical_structure Antigens Surface CD4 Antigens medicine Animals Humans Intracellular Immunobiology
Zdroj:	Blood. 118:1175-1176
ISSN:	1528-0020 0006-4971
Popis:	CC Chemokine Receptor 5 (CCR5) is an important mediator of chemotaxis and the primary coreceptor for HIV-1. A recent report by other researchers suggested that primary T cells harbor pools of intracellular CCR5. With the use of a series of complementary techniques to measure CCR5 expression (antibody labeling, Western blot, quantitative reverse transcription polymerase chain reaction), we established that intracellular pools of CCR5 do not exist and that the results obtained by the other researchers were false-positives that arose because of the generation of irrelevant binding sites for anti-CCR5 antibodies during fixation and permeabilization of cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1b9a96aabedc6e372f0160cbfa59a5da https://doi.org/10.1182/blood-2011-02-330886 Zobrazit plný text záznamu