Complete genome sequence and phylogenetic analysis of a novel dicistrovirus associated with the whitefly Bemisia tabaci
| Autor: | Vivian S. Lucena, Alice K. Inoue-Nagata, Tatsuya Nagata, Miguel Michereff-Filho, Marcio Hedil, Erich Y.T. Nakasu |
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| Rok vydání: | 2019 |
| Předmět: |
Cancer Research
food.ingredient viruses Sequence Homology Genome Viral Cripavirus Genome Hemiptera Open Reading Frames 03 medical and health sciences food Virology Animals Cluster Analysis Insect virus Cricket paralysis virus Phylogeny 030304 developmental biology Whole genome sequencing Genetics Dicistroviridae 0303 health sciences biology Reverse Transcriptase Polymerase Chain Reaction 030306 microbiology Computational Biology High-Throughput Nucleotide Sequencing RNA virus Sequence Analysis DNA biology.organism_classification Infectious Diseases Aparavirus |
| Zdroj: | Virus Research. 260:49-52 |
| ISSN: | 0168-1702 |
| DOI: | 10.1016/j.virusres.2018.11.008 |
| Popis: | A novel single-stranded RNA virus was detected in a whitefly (Bemisia tabaci) sample subjected to high-throughput sequencing. The 8293 nt-long genome presents a polyadenylated 3' end, and contains two ORFs encoding putative 1596 and 849 aa-long proteins. These putative proteins display significant similarity to replicase and capsid polyproteins, respectively, of discitroviruses. Its complete genome sequence shared the highest nucleotide identity (59.8%) with cricket paralysis virus (family Dicistroviridae, genus Cripavirus). Phylogenetic analyses showed that this new virus putative protein sequences clustered with those from members of Dicistroviridae. However, the replicase and capsid polyprotein sequences clustered with those of members of different genera, respectively to Aparavirus and Cripavirus. RT-PCR using newly collected adult and nymph whitefly samples confirmed the presence of this virus in field populations of B. tabaci. Genome sequence and organization, and polyproteins comparison indicate that this virus is a new species of the family Dicistroviridae. The name Bemisia-associated dicistrovirus 1 is proposed for this virus. |
| Databáze: | OpenAIRE |
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