Engulfment, persistence and fate of Bdellovibrio bacteriovorus predators inside human phagocytic cells informs their future therapeutic potential
| Autor: | Rob Till, Christopher Gell, Luisa Martinez-Pomares, Sally P. Wheatley, Christopher I. Moore, Patrick J. Tighe, R. Elizabeth Sockett, Jess Tyson, Paul M. Radford, Dhaarini Raghunathan, David Negus |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Cell Survival media_common.quotation_subject Population lcsh:Medicine medicine.disease_cause Microtubules Article Predation Bdellovibrio 03 medical and health sciences 0302 clinical medicine Phagosomes medicine Humans education lcsh:Science Cells Cultured media_common education.field_of_study Phagocytes Multidisciplinary biology lcsh:R Longevity Pathogenic bacteria U937 Cells Bdellovibrio bacteriovorus biology.organism_classification Actins Cell biology 030104 developmental biology lcsh:Q 030217 neurology & neurosurgery Bacteria Intracellular |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-16 (2019) |
| ISSN: | 2045-2322 |
| Popis: | In assessing the potential of predatory bacteria, such as Bdellovibrio bacteriovorus, to become live therapeutic agents against bacterial infections, it is crucial to understand and quantify Bdellovibrio host cell interactions at a molecular level. Here, we quantify the interactions of live B. bacteriovorus with human phagocytic cells, determining the uptake mechanisms, persistence, associated cytokine responses and intracellular trafficking of the non-growing B. bacteriovorus in PMA-differentiated U937 cells. B. bacteriovorus are engulfed by U937 cells and persist for 24 h without affecting host cell viability and can be observed microscopically and recovered and cultured post-uptake. The uptake of predators is passive and depends on the dynamics of the host cell cytoskeleton; the engulfed predators are eventually trafficked through the phagolysosomal pathway of degradation. We have also studied the prevalence of B. bacteriovorus specific antibodies in the general human population. Together, these results quantify a period of viable persistence and the ultimate fate of B. bacteriovorus inside phagocytic cells. They provide new knowledge on predator availability inside hosts, plus potential longevity and therefore potential efficacy as a treatment in humans and open up future fields of work testing if predators can prey on host-engulfed pathogenic bacteria. |
| Databáze: | OpenAIRE |
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