Osmotically Driven and Detected DNA Translocations
| Autor: | Angus McMullen, George Araujo, Derek Stein, Michele Winter |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Work (thermodynamics)
Osmosis Salinity Time Factors Diffusion lcsh:Medicine Chromosomal translocation 02 engineering and technology 01 natural sciences Article chemistry.chemical_compound Nanopores Electricity 0103 physical sciences A-DNA lcsh:Science 010306 general physics Multidisciplinary Chemistry Physics lcsh:R DNA 021001 nanoscience & nanotechnology Nanopore Biophysics lcsh:Q Current (fluid) 0210 nano-technology |
| Zdroj: | Scientific Reports Scientific Reports, Vol 9, Iss 1, Pp 1-10 (2019) |
| ISSN: | 2045-2322 |
| Popis: | A salinity gradient propels a DNA molecule through a solid-state nanopore and generates an ionic current whose change allows for the detection of the translocation. Measurements and theoretical analyses reveal the role of diffusio-osmosis in driving these phenomena: After accounting for known salinity-dependent electrode effects, the measured current change caused by the presence of a DNA molecule inside the nanopore and the DNA translocation speed through it both increase with the magnitude of the applied salinity gradients. The effects are consistent with the theory of diffuisio-osmosis and strong enough to enable DNA translocations to overcome an applied retarding potential of tens of millivolts. This work illustrates how salinity gradients can be used to power and operate a nanopore sensor. |
| Databáze: | OpenAIRE |
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