Influence of ovarian cancer type I and type II microenvironment on the phenotype and function of monocyte-derived dendritic cells

Autor: Wiesława Bednarek, Rafał Tarkowski, Karolina Okła, Iwona Wertel, Justyna Surówka, Jan Kotarski
Jazyk: angličtina
Rok vydání: 2017
Předmět:
0301 basic medicine
Cancer Research
Dendritic cells
T-Lymphocytes
Regulatory
Monocytes
Peritoneal fluid
0302 clinical medicine
Tumor Cells
Cultured
Tumor Microenvironment
Aged
80 and over
Ovarian Neoplasms
hemic and immune systems
Cell Differentiation
General Medicine
Regulatory T cells
Middle Aged
Adenocarcinoma
Mucinous
Interleukin-10
medicine.anatomical_structure
Phenotype
Oncology
030220 oncology & carcinogenesis
Female
Research Article
Adult
Regulatory T cell
CD14
chemical and pharmacologic phenomena
Biology
03 medical and health sciences
Young Adult
Immune system
Antigen
Ovarian cancer
Antigens
CD
medicine
Humans
Aged
CD86
Tumor microenvironment
Dendritic cell
medicine.disease
Cell cultures
Cystadenocarcinoma
Serous
Endometrial Neoplasms
030104 developmental biology
Immunology
Cancer research
Follow-Up Studies
Zdroj: Clinical & Translational Oncology
ISSN: 1699-3055
1699-048X
Popis: PURPOSE The aim of this study was to evaluate the influence of ovarian cancer cell lysates isolated from type I or type II ovarian cancer (OC) on the phenotype of monocyte-derived dendritic cells (Mo-DCs) and the cytokine profile. We also determined whether the Mo-DCs and tumor microenvironment, reflected by peritoneal fluid (PF) from type I or II ovarian cancer, could promote regulatory T cell (Tregs) differentiation from naive CD4+ lymphocytes in vitro. RESULTS Our results show a significant role of the ovarian cancer microenvironment reflected by PF from type I or II OC in the inhibition of the DC differentiation process. Interestingly, the percentage of cells co-expressing CD45 and CD14 antigens in the cultures stimulated with PF from both type I and type II OC was higher than in the control. Furthermore, the percentage of cells expressing CD1a, i.e., a marker of immature DCs, was significantly reduced in the cultures stimulated with PF from type I and type II OC. The results obtained show that ovarian cancer type II lysates induce differentiation of monocytes into macrophage-like cells with a CD1a+/HLA-DR+/CD83− phenotype and significantly higher CD86/HLA-DR expression. We show that ovarian cancer type II Mo-DCs are able to prevent an immune response by release of IL-10, whereas OC type I Mo-DCs can promote the generation of Tregs. CONCLUSIONS We demonstrate that each type of ovarian cancer can induce a unique phenotype of DCs and differentiation of Tregs, both associated with immune-suppressive function, which may be an obstacle while developing effective anticancer dendritic cell vaccination.
Databáze: OpenAIRE
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