Susceptibility of placental mitochondria to oxidative stress
| Autor: | María Gabriela Rovedatti, Rodrigo Papa Gobbi, Gladis Magnarelli |
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| Rok vydání: | 2018 |
| Předmět: |
Adult
0301 basic medicine Embryology medicine.medical_specialty Antioxidant Placenta Health Toxicology and Mutagenesis Protein Carbonylation medicine.medical_treatment Argentina Mitochondrion Toxicology medicine.disease_cause Antioxidants Lipid peroxidation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Pregnancy Internal medicine medicine Humans Organelles chemistry.chemical_classification Reactive oxygen species biology Chemistry Glutathione Catalase Mitochondria Oxidative Stress 030104 developmental biology Endocrinology 030220 oncology & carcinogenesis Pediatrics Perinatology and Child Health biology.protein Female Lipid Peroxidation Reactive Oxygen Species Oxidative stress Developmental Biology |
| Zdroj: | Birth Defects Research. 110:1228-1232 |
| ISSN: | 2472-1727 |
| Popis: | Background Two different mitochondrial fractions (MFs) have been characterized in the human placenta: the "light" and "heavy" fractions (LMF and HMF). Although these organelles are the main source of reactive oxygen species, an imbalance between their production and the rate of detoxification represents a serious threat to mitochondrial homeostasis and, in the case of the placenta, also to the fetus. The aim of this study was to evaluate the antioxidant capacity and susceptibility to oxidative stress in both types of MFs. Methods Human MFs were isolated from healthy donors (n = 11) and either incubated or not with H2 O2 . Catalase (CAT) activity, and reduced glutathione (GSH), lipid peroxidation (LP), and protein carbonylation (PC) levels were determined. Results H2 O2 treatment increased LP and PC levels and decreased CAT activity. GSH levels were similar in control and treated MFs. Conclusion H2 O2 caused oxidative damage in both LMF and HMF and the antioxidant system measured in these two MFs responded similarly. To the best of our knowledge, this is the first partial description of the antioxidant defense in placental HMF and LMF performed in a cell-free assay. The small number of antioxidant system parameters measured did not allow detecting differences between HMF and LMF. |
| Databáze: | OpenAIRE |
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