Genome-Wide Identification of Key Components of RNA Silencing in Two Phaseolus vulgaris Genotypes of Contrasting Origin and Their Expression Analyses in Response to Fungal Infection
| Autor: | Juan C. Alvarez-Diaz, Manon M. S. Richard, Vincent Thareau, Gianluca Teano, Christine Paysant-Le-Roux, Guillem Rigaill, Stéphanie Pflieger, Ariane Gratias, Valérie Geffroy |
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| Rok vydání: | 2021 |
| Předmět: |
QH426-470
Phaseolus vulgaris Article Colletotrichum lindemuthianum Gene Expression Regulation Plant Colletotrichum Genetics RNA silencing Argonaute double-stranded RNA binding (DRB) RNA-dependent RNA polymerase (RDR) Pol IV Phylogeny Genetics (clinical) Plant Diseases Plant Proteins Phaseolus DNA-Directed RNA Polymerases RNA-Dependent RNA Polymerase Argonaute Proteins RNA Interference Transcriptome Genome-Wide Association Study |
| Zdroj: | Genes Genes, Vol 13, Iss 64, p 64 (2022) Genes; Volume 13; Issue 1; Pages: 64 |
| ISSN: | 2073-4425 |
| DOI: | 10.3390/genes13010064 |
| Popis: | RNA silencing serves key roles in a multitude of cellular processes, including development, stress responses, metabolism, and maintenance of genome integrity. Dicer, Argonaute (AGO), double-stranded RNA binding (DRB) proteins, RNA-dependent RNA polymerase (RDR), and DNA-dependent RNA polymerases known as Pol IV and Pol V form core components to trigger RNA silencing. Common bean (Phaseolus vulgaris) is an important staple crop worldwide. In this study, we aimed to unravel the components of the RNA-guided silencing pathway in this non-model plant, taking advantage of the availability of two genome assemblies of Andean and Meso-American origin. We identified six PvDCLs, thirteen PvAGOs, 10 PvDRBs, 5 PvRDRs, in both genotypes, suggesting no recent gene amplification or deletion after the gene pool separation. In addition, we identified one PvNRPD1 and one PvNRPE1 encoding the largest subunits of Pol IV and Pol V, respectively. These genes were categorized into subgroups based on phylogenetic analyses. Comprehensive analyses of gene structure, genomic localization, and similarity among these genes were performed. Their expression patterns were investigated by means of expression models in different organs using online data and quantitative RT-PCR after pathogen infection. Several of the candidate genes were up-regulated after infection with the fungus Colletotrichum lindemuthianum. |
| Databáze: | OpenAIRE |
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