New alleles and mutational events at 14 STR loci from different German populations
| Autor: | Matthias Muche, Werner Brabetz, Lotte Henke, Karolin Hoppe, Martin Jung, Jeanett Edelmann, Frank Götz, Sandra Hering, Reinhard Szibor, Carsten Hohoff, Michael Klintschar, Burkhard Rolf, Klaus Bender, Dorit Becker, Volker Weirich |
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| Rok vydání: | 2007 |
| Předmět: |
Genetics
Forensic Genetics Male Mutation rate Base Sequence STR multiplex system DNA Biology Null allele Pathology and Forensic Medicine Genetics Population STR analysis Gene Frequency Germany Mutation Microsatellite Humans Female Primer (molecular biology) Allele Primer binding site Alleles DNA Primers Microsatellite Repeats |
| Zdroj: | Forensic science international. Genetics. 1(3-4) |
| ISSN: | 1878-0326 |
| Popis: | The molecular origin of DNA mutations and the mutation rates were analyzed at 14 short tandem repeat (STR) loci with samples from trio cases derived from 10 different German population samples. STR loci comprised of D2S1360, D3S1744, D4S2366, D5S2500, D6S474, D7S1517, D8S1132, D10S2325, D12S391, D18S51, D19S246, D20S480, D21S226, and D22S689. In a total of 488 meioses, 16 isolated genetic inconsistencies in 8 different STRs were observed, whereas no mutations were found at the other loci. The data of five mutations suggested the presence of silent or null alleles due to sequence variation in primer binding site. This could be confirmed for four suspected cases by the use of alternative primer sets and by DNA sequence analyses. Furthermore, this study revealed nine new allelic variants at five different loci. |
| Databáze: | OpenAIRE |
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