A conventional PCR for differentiating common taeniid species of dogs based on in silico microsatellite analysis
| Autor: | Balal Sadeghi, Saeedeh Shamsaddini, Majid Fasihi Harandi, Mansoureh Dehghani, Mohammad Ali Mohammadi, Sima Rostami, Seyed Reza Mirbadie |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Mitochondrial DNA lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 In silico Locus (genetics) Biology Polymerase Chain Reaction law.invention 03 medical and health sciences Dogs Mitochondrial genome Tandem repeat law parasitic diseases Animals Internal Transcribed Spacer Canine Tapeworms Polymerase chain reaction DNA Primers Genetics Sheep Taenia Microsatellite General Medicine 030108 mycology & parasitology biology.organism_classification Mitochondria Echinococcus Infectious Diseases Taeniidae Genetic marker Original Article Microsatellite Repeats |
| Zdroj: | Revista do Instituto de Medicina Tropical de São Paulo, Vol 59, Iss 0 (2017) Revista do Instituto de Medicina Tropical de São Paulo; Vol. 59 (2017); e66 Revista do Instituto de Medicina Tropical de São Paulo; v. 59 (2017); e66 Revista do Instituto de Medicina Tropical de São Paulo Instituto de Medicina Tropical (IMT) instacron:IMT Revista do Instituto de Medicina Tropical de São Paulo, Volume: 59, Article number: e66, Published: 04 SEP 2017 |
| ISSN: | 0036-4665 1678-9946 |
| DOI: | 10.1590/s1678-9946201759066 |
| Popis: | Canine taeniids are among the major tapeworms with remarkable medical and economic significance. Reliable diagnosis and differentiation of dog taeniids using simple and sensitive tools are of paramount importance for establishing an efficient surveillance system. Microsatellites as abundant unique tandem repeats of short DNA motifs are useful genetic markers for molecular epidemiological studies. The purpose of the present study was to find a primer pair for rapid differentiation of major tapeworms of dogs, Taenia hydatigena, T. multiceps, T. ovis and Echinococcus granulosus, by screening existing nucleotide data. All the mitochondrial genome records as well as non-coding ITS1 sequences of Taeniidae species were downloaded from Nucleotide database from NCBI. For prediction and analysis of potential loci of STR/SSR in ITS1 as well as mitochondrial regions, we used ChloroMitoSSRDB 2.0 and GMATo v1.2. software. Different tapeworm species were categorized according to different motif sequences and type and size of each microsatellite locus. Three primer sets were designed and tested for differentiating taeniid species and evaluated in a conventional PCR system. Four taeniid species were successfully differentiated using a primer pair in a simple conventional PCR system. We predicted 2-19 and 1-4 microsatellite loci in ITS1 and mitochondrial genome, respectively. In ITS1, 41 Di and 21 Tri motifs were found in the taeniids while the majority of the motifs in the mitochondrial genome were Tetra (89) and Tri (70). It is documented that the number and diversity of microsatellite loci is higher in nuclear ITS1 region than mostly coding mitochondrial genome. |
| Databáze: | OpenAIRE |
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