Analysis of Stress Responsive Genes Induced by Single-Walled Carbon Nanotubes in BJ Foreskin Cells
Autor: | Renard L. Thomas, Enrique V. Barrera, Allison C. Rice-Ficht, Olufisayo Jejelowo, Shubhashish Sarkar, Govindarajan T. Ramesh, Chidananda S. Sharma, Rajeshwari Yog, Bobby L. Wilson, Adaikkappan Periakaruppan |
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Rok vydání: | 2007 |
Předmět: |
Keratinocytes
Male HMOX1 Materials science Foreskin Biomedical Engineering Gene Expression Bioengineering Nanotechnology Carbon nanotube medicine.disease_cause Article Cell Line law.invention Nanomaterials law medicine Humans General Materials Science chemistry.chemical_classification Reactive oxygen species Nanotubes Carbon Reverse Transcriptase Polymerase Chain Reaction Dimethylformamide General Chemistry Condensed Matter Physics Cell biology Kinetics Oxidative Stress medicine.anatomical_structure chemistry Cell culture Solvents Signal transduction Reactive Oxygen Species Oxidative stress |
Zdroj: | Journal of Nanoscience and Nanotechnology. 7:584-592 |
ISSN: | 1533-4880 |
Popis: | Nanotechnology is finding its use as a potential technology in consumer products, defense, electronics, and medical applications by exploiting the properties of nanomaterials. Single-walled carbon nanotubes are novel forms of these nanomaterials with potential for large applications. However, the toxicity studies on this material are not explored in detail and therefore limiting its use. It has been earlier reported that single-walled carbon nanotubes induces oxidative stress and also dictates activation of specific signaling pathway in keratinocytes. The present study explores the effect of single-walled carbon nanotubes on stress genes in human BJ Foreskin cells. The results show induction of oxidative stress in BJ Foreskin cells by single-walled carbon nanotubes and increase in stress responsive genes. The genes included inducible genes like HMOX1, HMOX2, and Cyp1B1. In addition we validated increase for four genes by SWCNT, namely ATM, CCNC, DNAJB4, and GADD45A by RT-PCR. Moreover results of the altered stress related genes have been discussed and that partially explains some of the toxic responses induced by single-walled carbon nanotubes. |
Databáze: | OpenAIRE |
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