Mosaic-Like Structure of Penicillin-Binding Protein 2 Gene ( penA ) in Clinical Isolates of Neisseria gonorrhoeae with Reduced Susceptibility to Cefixime
| Autor: | Yukihiko Oishi, Masahiro Takahata, Satoshi Ameyama, Shoichi Onodera, Nobuko Maki, Shinzaburo Minami, Katsuhisa Endo, Hiroo Suzuki, Hirokazu Goto |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
Male
Penicillin binding proteins Molecular Sequence Data Microbial Sensitivity Tests Neisseria flavescens Muramoylpentapeptide Carboxypeptidase medicine.disease_cause Microbiology Bacterial Proteins Cefixime Multienzyme Complexes Mechanisms of Resistance medicine Humans Penicillin-Binding Proteins Pharmacology (medical) Amino Acid Sequence Pharmacology biology Mosaicism Neisseria meningitidis Genetic transfer biology.organism_classification Virology Neisseria gonorrhoeae Penicillin Neisseria cinerea Infectious Diseases Hexosyltransferases Peptidyl Transferases Carrier Proteins medicine.drug |
| Zdroj: | Antimicrobial Agents and Chemotherapy. 46:3744-3749 |
| ISSN: | 1098-6596 0066-4804 |
| DOI: | 10.1128/aac.46.12.3744-3749.2002 |
| Popis: | Neisseria gonorrhoeae strains with reduced susceptibility to cefixime (MICs, 0.25 to 0.5 μg/ml) were isolated from male urethritis patients in Tokyo, Japan, in 2000 and 2001. The resistance to cephems including cefixime and penicillin was transferred to a susceptible recipient, N. gonorrhoeae ATCC 19424, by transformation of the penicillin-binding protein 2 gene ( penA ) that had been amplified by PCR from a strain with reduced susceptibility to cefixime (MIC, 0.5 μg/ml). The sequences of penA in the strains with reduced susceptibilities to cefixime were different from those of other susceptible isolates and did not correspond to the reported N. gonorrhoeae penA gene sequences. Some regions in the transpeptidase-encoding domain in this penA gene were similar to those in the penA genes of Neisseria perflava ( N. sicca ), Neisseria cinerea , Neisseria flavescens , and Neisseria meningitidis . These results showed that a mosaic-like structure in the penA gene conferred reductions in the levels of susceptibility of N. gonorrhoeae to cephems and penicillin in a manner similar to that found for N. meningitidis and Streptococcus pneumoniae . |
| Databáze: | OpenAIRE |
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