Expression of Egr1 and p53 in human carotid plaques and apoptosis induced by 7-oxysterol or p53
| Autor: | Sayem Miah, Xi-Ming Yuan, Wei Li, Shahram Nour Mohammad Zadeh |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
endocrine system
Pathology medicine.medical_specialty Oxysterol Cell Culture Techniques EGR1 Apoptosis Biology Toxicology Pathology and Forensic Medicine Mice medicine Animals Humans Carotid Stenosis Fragmentation (cell biology) Ketocholesterols Early Growth Response Protein 1 chemistry.chemical_classification Reactive oxygen species U937 cell U937 Cells Cell Biology General Medicine medicine.disease Immunohistochemistry Hydroxycholesterols Atheroma chemistry Cell culture Cancer research lipids (amino acids peptides and proteins) Tumor Suppressor Protein p53 Reactive Oxygen Species |
| Zdroj: | Experimental and Toxicologic Pathology. 65:677-682 |
| ISSN: | 0940-2993 |
| DOI: | 10.1016/j.etp.2012.08.002 |
| Popis: | Egr-1 and p53 are involved in pathology of both atherosclerosis and cancer. However, it is unknown whether p53 and Egr1 are interactively involved in apoptosis in atherosclerosis. We found that in human carotid plaques, the expression of p53 was inversely correlated with Egr1. In U937 cells, 7β-hydroxycholesterol and 7-ketocholesterol induced production of reactive oxygen species (ROS), transient up-regulation of Egr1 followed by late induction of p53 and apoptosis. Cells with nuclear fragmentation induced by 7-oxysterol or p53 showed increased levels of p53, but decreased levels of Egr1. In conclusion, ROS induced by 7-oxysterols may function as an early initiator of Egr1 expression. The late induced p53 by 7-oxysterols contributes to apoptotic cell death and is linked to the reduction of Egr1 levels, which resembles the differential expression of p53 and Egr1 in human atheroma progression. |
| Databáze: | OpenAIRE |
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