Recruitment of Plasma Membrane GABA-A Receptors by Submembranous Gephyrin/Collybistin Clusters
| Autor: | Angel L. De Blas, Karthik Kanamalla, Shanu George, Tzu-Ting Chiou |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
0301 basic medicine
Protein subunit AMPA receptor Receptors N-Methyl-D-Aspartate 03 medical and health sciences Cellular and Molecular Neuroscience 0302 clinical medicine Postsynaptic potential Humans gamma-Aminobutyric Acid biology Gephyrin Chemistry GABAA receptor Cell Membrane Glutamate receptor Membrane Proteins Cell Biology General Medicine Receptors GABA-A HEK293 Cells 030104 developmental biology Synapses biology.protein Biophysics NMDA receptor Collybistin Rho Guanine Nucleotide Exchange Factors 030217 neurology & neurosurgery |
| Zdroj: | Cellular and Molecular Neurobiology. 42:1585-1604 |
| ISSN: | 1573-6830 0272-4340 |
| DOI: | 10.1007/s10571-021-01050-1 |
| Popis: | It has been shown that subunit composition is the main determinant of the synaptic or extrasynaptic localization of GABAA receptors (GABAARs). Synaptic and extrasynaptic GABAARs are involved in phasic and tonic inhibition, respectively. It has been proposed that synaptic GABAARs bind to the postsynaptic gephyrin/collybistin (Geph/CB) lattice, but not the typically extrasynaptic GABAARs. Nevertheless, there are no studies of the direct binding of various types of GABAARs with the submembranous Geph/CB lattice in the absence of other synaptic proteins, some of which are known to interact with GABAARs. We have reconstituted GABAARs of various subunit compositions, together with the Geph/CB scaffold, in HEK293 cells, and have investigated the recruitment of surface GABAARs by submembranous Geph/CB clusters. Results show that the typically synaptic α1β3γ2 GABAARs were trapped by submembranous Geph/CB clusters. The α5β3γ2 GABAARs, which are both synaptic and extrasynaptic, were also trapped by Geph/CB clusters. Extrasynaptic α4β3δ GABAARs consistently showed little or no trapping by the Geph/CB clusters. However, the extrasynaptic α6β3δ, α1β3, α6β3 (and less α4β3) GABAARs were highly trapped by the Geph/CB clusters. AMPA and NMDA glutamate receptors were not trapped. The results suggest: (I) in the absence of other synaptic molecules, the Geph/CB lattice has the capacity to trap not only synaptic but also several typically extrasynaptic GABAARs; (II) the Geph/CB lattice is important but does not play a decisive role in the synaptic localization of GABAARs; and (III) in neurons there must be mechanisms preventing the trapping of several typically extrasynaptic GABAARs by the postsynaptic Geph/CB lattice. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink