Interleukin-1β Regulation of Adhesion Molecules on Human Gingival and Periodontal Ligament Fibroblasts
| Autor: | Brandon H. Joe, Philip J. Hanes, Meral Keskintepe, Jason M. Mailhot, Baldev B. Singh, James L. Borke |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Pathology
medicine.medical_specialty Periodontal Ligament Gingiva Fluorescent Antibody Technique Vascular Cell Adhesion Molecule-1 Antibodies Pathogenesis Humans Medicine Periodontal fiber Least-Squares Analysis Cell adhesion Cells Cultured Periodontal Diseases Fluorescent Dyes Analysis of Variance business.industry Cell adhesion molecule General Engineering Soluble cell adhesion molecules Interleukin Adhesion Fibroblasts Flow Cytometry Intercellular Adhesion Molecule-1 Intercellular adhesion molecule Up-Regulation Linear Models Periodontics business Cell Adhesion Molecules Fluorescein-5-isothiocyanate Interleukin-1 |
| Zdroj: | Journal of Periodontology. 72:865-870 |
| ISSN: | 1943-3670 0022-3492 |
| Popis: | Adhesion molecules have been implicated in the pathogenesis of rheumatoid arthritis and may also play a role in the pathogenesis of periodontal disease by promoting the recruitment and retention of leukocytes in gingival tissue.The aim of the present study was to evaluate the capacity of interleukin-1beta (IL-1beta) to regulate adhesion molecule expression on clinically healthy human gingival (HGF) and periodontal ligament (PDL) fibroblasts. The HGF (n = 6) and PDL (n = 3) fibroblasts were treated with 1.0 ng/ml of IL-1beta for 24 hours and then incubated with primary intercellular adhesion molecule-1 (ICAM-1) and vascular cellular adhesion molecule-1 (VCAM-1) antibodies followed by FITC-conjugated secondary antibodies. The expression of ICAM-1 and VCAM-1 was measured by immunofluorescence flow cytometry.The levels of ICAM-1 expression in IL-1beta treated HGF and PDL fibroblasts were statistically significant (Por = 0.05) compared to normal untreated controls using log-transformed data and 3-way analysis of variance. Both cells expressed VCAM-1 after IL-1beta treatment, but the levels were not statistically different from controls.This study demonstrated that IL-1beta upregulated ICAM-1 expression in both HGF and PDL fibroblasts. Even though the level of VCAM-1 was not statistically different from both HGF and PDL fibroblasts treated with IL-1beta compared to controls, both cells do express the VCAM-1 molecules. These results suggest that ICAM-1 and VCAM-1 might be involved in the pathogenesis of periodontal disease. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text