Dimensional assessment of X-bearing and Y-bearing haploid and disomic human sperm with the use of fluorescence in situ hybridization and objective morphometry
| Autor: | Attila Jakab, David C. Ward, Ciler Celik-Ozenci, Zoltan Zavaczki, Gabor Huszar, Laszlo Ovari, G. Leyla Sati |
|---|---|
| Rok vydání: | 2006 |
| Předmět: |
Male
Short axis Genotype Sperm Head Semen Haploidy Biology Andrology Reproductive biology medicine Humans Microscopy Phase-Contrast Cell Shape In Situ Hybridization Fluorescence Genetics Chromosomes Human X Chromosomes Human Y medicine.diagnostic_test Outcome measures Obstetrics and Gynecology Oligospermia Uniparental Disomy Spermatozoa Sperm Reproductive Medicine Sperm Tail Ploidy Fluorescence in situ hybridization |
| Zdroj: | Fertility and Sterility. 85:121-127 |
| ISSN: | 0015-0282 |
| DOI: | 10.1016/j.fertnstert.2005.07.1295 |
| Popis: | Objective To explore the dimensional attributes of haploid and disomic X-bearing and Y-bearing spermatozoa. Design Morphometric evaluation of more than 2,000 X-bearing and Y-bearing spermatozoa after identification of the genotype with fluorescence in situ hybridization. Setting Academic clinical and research andrology laboratory. Main Outcome Measure(s) Sperm head area, perimeter, long axis, short axis, shape factor, elliptical form factor (long axis/short axis), and tail length. Result(s) We found no differences in dimensions or dimensional distributions between X-bearing and Y-bearing spermatozoa, whether in the native or the decondensed state, or in oligozoospermic or normozoospermic men. There were inconsistent differences and a 70% overlap in the dimensions of haploid and disomic spermatozoa. The other 30% of sperm with disomic nuclei were either smaller or larger compared to haploid spermatozoa. Conclusion(s) There are no differences, or distinguishing characteristics, in dimensions or dimensional distributions between X-bearing and Y-bearing spermatozoa. Dimensional attributes do not discriminate between dysomic and haploid spermatozoa. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|